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Molecular Probes™ LIVE/DEAD™ FungaLight™ Yeast Viability Kit, for flow cytometry

The LIVE/DEAD FungaLight Yeast Viability Kit enables researchers to easily, reliably, and quantitatively distinguish live and dead yeast in minutes by flow cytometry.

Brand:  Molecular Probes™ L34952

Product Code. 10423262

  • 878.00 € / Each

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For Research Use Only. All usage must comply with product instructions.

This item is not returnable. View return policy

Description

Description

The LIVE/DEAD™ FungaLight™ Yeast Viability Kit enables researchers to easily, reliably and quantitatively distinguish live and dead yeast in minutes by flow cytometry. The kit contains solutions of the SYTO 9 green-fluorescent nucleic acid stain and the red-fluorescent nucleic acid stain, propidium iodide. These stains differ both in their spectral characteristics and in their ability to penetrate healthy yeast cells. When used alone, the SYTO 9 stain generally labels all yeast in a population - those with intact membranes and those with damaged membranes. In contrast, propidium iodide penetrates only yeast with damaged membranes, causing a reduction in the SYTO 9 stain fluorescence by fluorescence resonance energy transfer (FRET) when both dyes are present. As a result, yeast with intact membranes stain fluorescent green, whereas yeast with damaged membranes stain fluorescent red.

Any physiological buffer between pH 7.0–8.0, including PBS, can be used to dilute the SYTO dyes for the staining solution.

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Impermeant, Permeant
Contains 1 vial of SYTO™ 9 dye (solution in DMSO), and 1 vial of propidium iodide, 300 μL (solution in DMSO).

Store in freezer (-5°C to -30°C) and protect from light.
Fluorescence
Flow Cytometer
SYTO™ 9, propidium iodide
485/535 nm
Tube(s)
200 Assays kit
Green, Red
LIVE/DEAD™ FungaLight™ Yeast Viability Kit, for flow cytometry
Viability Assay
Yeast Viability Kit
498/617 nm
Solution
FungaLight™, LIVE/DEAD™
Room Temperature
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For Research Use Only. Not for use in diagnostic procedures.