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LNCaP clone FGC (human prostate carcinoma) Whole cell lysate, Denatured; Abnova

Product Code. 16079495
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Quantity:
200 μg
Unit Size:
200µg
This item is not returnable. View return policy

Product Code. 16079495

Brand: Abnova L004V1.200ug

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This item is not returnable. View return policy

Description

  • Whole cell lysate (denatured)
  • Tissue: prostate
  • Host: Human
  • Preparation: homogenization in ice-cold modified RIPA Lysis
  • Lysis buffer: 50mM Tris-HCl pH 7.4, 150mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% sodium deoxycholate, 1mM PMSF
  • Storage buffer: in ready-to-use 1X sample buffer (50mM Tris-HCl, 2% SDS, 10% glycerol, 300mM 2-mercaptoethanol, 0.01% bromophenol blue)

Western blotting

Specifications

For Use With (Application) Western Blot
Tissue Prostate
Description Whole cell lysate (denatured)
Lysis Buffer Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Preparation Method Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was mixed in 5X Sample Buffer to become final 1X Sample Buffer as Storage Buffer. The lysate was heated at 95°C for 5 min, and cooled rapidly.
Quality Control Testing 12.5% SDS-PAGE Stained with Coomassie Blue.
Quantity 200 μg
Storage Buffer In ready-to-use 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue).
Host Species Human
Concentration 3 mg/mL
Content And Storage Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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