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  7. 30245142

Invitrogen™ Lyo-ready Enzymes and Reagents for Recombinase Polymerase Amplification (RPA)

Invitrogen Lyo-ready RPA reagents are designed for fast, sensitive, and specific DNA amplification through isothermal recombinase polymerase amplification (RPA) reactions. Lyo-ready RPA reagents are available in stand-alone and kit formats.

Brand:  Invitrogen™ A72127

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Product Code. 30245142

  • 251.00 € / 100 reactions

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Description

Description

Invitrogen Lyo-ready recombinase polymerase amplification (RPA) reagents are designed for fast, sensitive, and specific DNA amplification through isothermal RPA reactions. Lyo-ready RPA reagents are available in stand-alone and kit formats. Reverse transcriptase can be added to the RPA reaction for amplification of RNA targets. All reagents are glycerol-free, which enables assay lyophilization.

Features of Lyo-ready RPA products

  • Enhanced sensitivity down to 1 copy using optimized (RT-)RPA reaction buffer
  • Robust and specific RNA/DNA amplification
  • Amplification from inhibitor-containing samples
  • Fast target amplification in as little as 20 minutes
  • Ability to easily optimize RPA or RT-RPA reactions

Kit components

The Lyo-ready RPA Kit includes all required components for an RPA reaction:

  • Lyo-ready T4 UvsX Protein—produced from E. coli cells bearing cloned uvsX gene derived from bacteriophage T4
  • Lyo-ready T4 UvsY Protein—a recombination mediator protein that stimulates homologous recombination performed by the UvsX recombinase
  • Lyo-ready Bst DNA Polymerase (Cat. No. A56655)—a proprietary mutant of Bst DNA Polymerase, Large Fragment, with fast reaction speed, increased sensitivity, and tolerance to inhibitors
  • Lyo-ready T4 Gene 32 Protein—a single-stranded DNA binding protein produced from E. coli cells carrying cloned bacteriophage T4 gene 32
  • Optimized reaction buffer, magnesium chloride, and dNTPs
TRUSTED_SUSTAINABILITY
Specifications

Specifications

Lyo-ready RPA Kit
RPA, RT-RPA
100 Reactions

• Lyo-ready T4 UvsX Protein (1.2 mg/mL), 50 μL
• Lyo-ready T4 UvsY Protein (1.2 mg/mL), 50 μL
• Lyo-ready Bst DNA Polymerase (6 U/μL), 50 μL
• Lyo-ready T4 Gene 32 Protein (16 mg/mL), 50 μL
• 2X RPA Reaction Buffer, 1000 μL
• 280 mM MgCl2, 100 μL
• 10 mM dNTP Mix, 50 μL

Store at -25°C to -15°C.
SuperScript IV Reverse Transcriptase
End point, real-time, lateral flow
Liquid
Lyo-ready Bst DNA Polymerase
Nucleic Acid Amplification
42°C
Dry Ice
Up to 20 min.
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Lot Number Certificate Type Date Catalog Number
Lot Number3151377 Certificate TypeCertificate of Analysis Date27/01/2025 Catalog NumberA72127
Lot Number3095716 Certificate TypeCertificate of Analysis Date27/01/2025 Catalog NumberA72127
Lot Number3080746 Certificate TypeCertificate of Analysis Date25/01/2025 Catalog NumberA72127
Lot Number3106443 Certificate TypeCertificate of Analysis Date25/01/2025 Catalog NumberA72127
Lot Number3106761 Certificate TypeCertificate of Analysis Date25/01/2025 Catalog NumberA72127
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Product Title
Invitrogen™ Lyo-ready Enzymes and Reagents for Recombinase Polymerase Amplification (RPA) > Product: Lyo-ready RPA Kit, Quantity: 100 Reactions, Type: DNA Amplification Kit

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