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Applied BioSystems™ MagMAX™ Saliva gDNA Isolation Kit

The MagMAX Saliva gDNA Isolation Kit is a scalable and automatable magnetic bead-based sample preparation technology for the isolation of genomic DNA (gDNA) from fresh and stabilized saliva.

381.00 € - 2230.00 €

Specifications

Format Kit
Isolation Technology Magnetic Bead
For Use With (Application) Next-Generation Sequencing, PCR
High-throughput Compatibility High-throughput Compatible, Manual Protocols, Automated Protocols
Product Line MagMAX™
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Products 4
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Product Code Brand No. of Reactions Price Quantity & Availability  
15930533
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Applied BioSystems™
A39059
100 This item is currently unavailable or has been discontinued.
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15900391
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Applied BioSystems™
A39059
100
499.00 €
Each
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15940533
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Applied BioSystems™
A39060
500 This item is currently unavailable or has been discontinued.
View the product page for possible alternatives.
N/A
15910391
View Documents
Applied BioSystems™
A39060
500
2230.00 €
Each
Please to purchase this item. Need a web account? Register with us today!
Description

Description

The MagMAX Saliva gDNA Isolation Kit is a scalable and automatable magnetic bead-based sample preparation technology for the isolation of genomic DNA (gDNA) from fresh and stabilized saliva. The resulting purified gDNA is ideal for a variety of downstream molecular biology applications such as qPCR, next-generation sequencing, and microarray analysis. All components are also available for purchase separately.

Key features of the MagMAX Saliva gDNA Isolation Kit:
• Process up to 96 samples in 25 minutes with 5 minutes hands-on time
• Isolate gDNA from 200 μL–2 mL of saliva using simple protocols in SBS microtiter plates
• Skip sample and buffer volume normalization steps for hassle-free processing
• Download validated BindIt software automation scripts for KingFisher Duo Prime and KingFisher Flex purification systems

Isolate DNA from stabilized and freshly collected saliva
The MagMAX Saliva gDNA Isolation Kit isolates gDNA from 200 μL–2 mL of freshly collected saliva or stabilized saliva collected using a commercially available saliva collection device (see figure below). MagMAX Saliva gDNA isolation chemistry is compatible with the Oragene™ saliva collection devices from DNA Genotek, the SalivaGard™ saliva collection device from Biomatrica, and the GeneFiX™ Saliva DNA Collection Device from IsoHelix.

The MagMAX Saliva gDNA Isolation Kit maximizes gDNA yields by treating samples with an innovative lysis/binding solution to simultaneously lyse cells, denature proteins, and capture gDNA on high-capacity magnetic binding beads. The Saliva gDNA Wash I Solution removes proteins, detergents, and other impurities from the bound nucleic acid. The gDNA is then released from the beads using a low-TE elution solution.

Fast and hassle-free magnetic bead-based workflows
The MagMAX Saliva gDNA Isolation Kit combines multiple laborious sample processing steps together into a single step to minimize the time and effort typically required by other methods, without compromising yield and purity. Process a broad range of sample volume inputs simultaneously without sample normalization or reagent volume adjustments for hassle-free sample prep. Simply match your saliva input volumes to one of three standard input workflows (50 μL to 500 μL, up to 1 mL, up to 2 mL) and follow the instructions for semi-automated sample preparation using the KingFisher Duo Prime or KingFisher Flex purification systems.

Optimized for KingFisher purification systems
The MagMAX Saliva gDNA Isolation Kit is optimized for use on the moderate-throughput KingFisher Duo Prime (Cat. No. 5400110) and the higher-throughput KingFisher Flex (Cat. No. 5400630) purification systems. Users can process up to 500 μL of saliva in high-throughput capacity SBS 96-well deep-well microtiter plates using the KingFisher Flex system. For smaller sample batches, users can process 12 samples using the Duo Prime system. Larger sample input volumes (>500 μL, and 1–2 mL), which are more challenging to process, are purified in the larger sample volume capacity 24-well deep-well SBS plates for 24 samples on the Flex or 6 samples on the Duo Prime system. KingFisher instrument BindIt software scripts for all sample types and both Flex and Duo Prime systems can be downloaded below and installed onto the KingFisher instruments.

Specifications

Specifications

Kit
Next-Generation Sequencing, PCR
MagMAX™
Room Temperature
25 min.
Magnetic Bead
High-throughput Compatible, Manual Protocols, Automated Protocols
Saliva gDNA Isolation Kit
Genomic DNA
Videos
SDS
Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.

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