Invitrogen™ Amplex™ Red Hydrogen Peroxide/Peroxidase Assay Kit

Description

Assay uses AmplexRed reagent (10-acetyl-3,7-dihydroxyphenoxazine) to detect hydrogen peroxide (H₂O₂) or peroxidase activity in vitro
. AmplexRed reagent, in combination with horseradish peroxidase (HRP), can detect H₂O₂ released from biological samples, including cells, or generated in enzyme-coupled reactions. AmplexRed reagent can also be used as an ultrasensitive assay for peroxidase activity when H₂O₂ is in excess. In the presence of peroxidase, AmplexRed reagent reacts with H₂O₂ in a 1:1 stoichiometry to produce the red-fluorescent oxidation product, resorufin. Resorufin has excitation and emission maxima of approximately 571 nm and 585 nm, respectively, and because the extinction coefficient is high (58,000 ± 5000cm–¹M–¹), the assay can be performed fluorometrically or spectrophotometrically. This reaction has been used to detect as little as 10 picomoles of H₂O₂ in a 100μL volume or 1 x 10–5U/mL of HRP.

• Can detect as little as 10 picomoles of hydrogen peroxide or 10μU/mL of horseradish peroxidase activity in a 100μL assay volume
• Provides sufficient reagents for approximately 500 assays using reaction volumes of 100μL per assay

Cell Analysis, Cell Culture, Cell Viability, Proliferation and Function, Enzyme and Protein Activity Assays, Nitro-Oxidative Stress, Oxidase and Peroxidase Activity, Proteins, Expression, Isolation and Analysis, Reactive Oxygen Species, Stem Cell Analysis, Stem Cell Labeling and Detection, Stem Cell Research

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