Invitrogen™ CellLight™ ER-GFP, BacMam 2.0

Description

This ready-to-use construct is transfected into cells using BacMam 2.0 technology and expresses GFP fused to the ER signal sequence of calreticulin and KDEL (ER retention signal) with minimal cellular disruption. You can observe ER-GFP behavior in live cells using fluorescent imaging and multiplex with other fluorescent proteins or organic dyes.

Cells expressing CellLight constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

BacMam Technology

CellLight ER-GFP, BacMam 2.0, is a fusion construct of ER signal sequence of calreticulin and KDEL (ER retention signal) and emGFP, providing accurate and specific targeting to cellular ER-GFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

• Fast and convenient: simply add CellLight reagent to cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
• Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
• Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
• Less toxic: CellLight reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling
• Flow Cytometer Laser Lines: 488

Cell Analysis, Cell Structure, Cell Tracing & Tracking, Cellular Imaging, Endoplasmic Reticulum (ER), Immunocytochemistry (ICC), Immunofluorescence (IF), Organelle Tracing

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