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Invitrogen™ CellLight™ Late Endosomes-RFP, BacMam 2.0
Provides easy way to label late endosomes with red fluorescent protein in live cells
Brand: Invitrogen™ C10589
401.35 EUR valid until 2024-12-14
Use promo code "21632" to get your promotional price.
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To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”21632” to get your promotional price
Description
This ready-to-use construct is transfected into cells using BacMam 2.0 technology, where it expresses RFP fused to Rab7a. You can observe late endosomes-RFP behavior in live cells independently of organelle pH and label with multiple tracking or tracing dyes to image dynamic cellular processes.
Cells expressing CellLight constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.
BacMam Technology
CellLight Late Endosomes-RFP, BacMam 2.0, is a fusion construct of Rab7a and TagRFP, providing accurate and specific targeting to cellular Late Endosomes-RFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.
- Fast and convenient: simply add CellLight reagent to cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
- Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
- Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
- Less toxic: CellLight reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling
Cell Analysis, Cell Structure, Cell Tracing & Tracking, Cellular Imaging, Endosomes, Immunofluorescence (IF), Organelle Tracing
Order Info
Shipping Condition: Wet Ice
Specifications
| Red-Orange | |
| RFP (TagRFP) | |
| 1 vial(s) | |
| Visible | |
| CellLight™, Molecular Probes™ | |
| Endocytosis | |
| Fluorescent Dye | |
| Endosomes |
| Store at 2°C to 6°C, protected from light. Do Not Freeze. | |
| Fluorescence Microscope, Flow Cytometer | |
| Fluorescence | |
| Liquid | |
| Wet Ice | |
| Fluorescence Intensity | |
| Endosomes Label |
For Research Use Only. Not for use in diagnostic procedures.