Substrates cleaved within cell for fluorescent product
Brand: Invitrogen™ I2904
793.44 EUR valid until 2025-03-28
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The ImaGene Green™ lacZ Gene Expression Kit contains a fluorescein-based galactosidase substrate that has been covalently modified to include a 12-carbon lipophilic moiety, C12FDG. Once inside the cell, the substrates are cleaved by β-galactosidase, producing a fluorescent product that is well retained by the cells, probably by incorporation of the lipophilic tail within the cellular membrane. In addition to the C12FDG, the kits also include the broad-spectrum β-galactosidase inhibitor, PETG, and chloroquine diphosphate for inhibiting acidic hydrolysis of the substrate.
Beta-Galactosidase Assays, Cell Analysis, Cellular Imaging, Enzyme and Protein Activity Assays, Flow Cytometry, Immunofluorescence (IF), Immunofluorescence Staining and Detection, Proteins, Expression, Isolation and Analysis, Reporter Assays
Shipping Condition: Room Temperature
| β-Galactosidase Reporter Gene Assay System | |
| β-Gal (lacZ) Assay | |
| Room Temperature | |
| Beta-Gal Substrate | |
| Beta-Galactosidase | |
| ImaGene Green |
| Fluorescence | |
| Store in freezer (-5 to -30°C) and protect from light. | |
| C12FDG | |
| Chemical Substrate | |
| Kit | |
| 1 kit |
Catalog No. 12559069
Catalog No. 12077549
Catalog No. 10729174
Catalog No. 11514270
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For Research Use Only. Not for use in diagnostic procedures.
