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Invitrogen™ SBFI, AM, cell permeant - Special Packaging

Sodium-sensitive molecule used to estimate Na+ gradients in isolated mitochondria, to measure intracellular NA+ levels, or to measure Na+ efflux in cells

Brand:  Invitrogen™ S1264

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Product Code. 11510666

  • 1282.00 € / 1000µg

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For Research Use Only. All usage must comply with product instructions.

This item is not returnable. View return policy

Description

Description

  • In combination with other fluorescent indicators used to correlate changes in intracellular Na+ with Ca2+ and Mg2+ concentrations, intracellular pH, and membrane potential
  • Although selectivity of SBFI for Na+ is less than that of calcium indicators such as fura-2, it is sufficient for detection of physiological concentrations of Na+ in presence of other monovalent cations
  • Spectral response of SBFI upon ion binding permit excitation ratio measurements, and this indicator can be used with same optical filters and equipment used for fura-
  • Label (Ex/Em): SBFI (approximately 340,380/500nm)
  • Lyophilized product may be dissolved in DMSO for use
  • Product is typically loaded into cells by adding dissolved indicator to medium containing cells
  • Selectivity Considerations and Cell Loading Strategies
  • Dissociation constant (Kd) of SBFI for Na+ is 3.8mm in absence of K+, and 11.3mm in solutions with combined Na+ and K+ concentration of 135mm (which approximates physiological ionic strength)
  • SBFI is approximately 18-fold more selective for Na+ than for K+

Cell Analysis, Cell Metabolism, Cell Viability, Proliferation and Function, Cell-Based Ion Channel Assays, Drug Discovery and Development, Ion Channel Biology, Ionic Homeostasis and Signaling, Sodium and Potassium Detection, Target and Lead Identification and Validation

Order Info

Shipping Condition: Room temperature

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Store in freezer -5°C to -30°C and protect from light.
Cell Viability and Proliferation
SBFI AM
20 x 50 μg
Fluorescence
Fluorescence Microscope
Sodium Indicator
Room Temperature
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5 results found
Lot Number Certificate Type Date Catalog Number
Lot Number2856059 Certificate TypeCertificate of Analysis Date06/06/2024 Catalog NumberS1264
Lot Number2680192 Certificate TypeCertificate of Analysis Date09/10/2023 Catalog NumberS1264
Lot Number2544365 Certificate TypeCertificate of Analysis Date28/09/2022 Catalog NumberS1264
Lot Number2286904 Certificate TypeCertificate of Analysis Date27/08/2021 Catalog NumberS1264
Lot Number2100972 Certificate TypeCertificate of Analysis Date01/04/2019 Catalog NumberS1264
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Invitrogen™ SBFI, AM, cell permeant - Special Packaging > Special Packaging; 20 x 50ug

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For Research Use Only. Not for use in diagnostic procedures.