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Proteintech™ Mouse CCL4 ELISA Kit
KE10030 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse CCL4 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse CCL4.
Brand: Proteintech™ KE10030-96T
This item is not returnable.
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Description
- CCL4(C-C chemokine ligand 4), also known as MIP-1β, plays a key role in inflammation and immune regulation.
- CCL4 is a major HIV-suppressive factor produced by CD8+ T cells. CCL4 is produced by a wide variety of cells including: immune cells (monocytes, B and T cells), fibroblasts, endothelial, and epithelial cells. CCL4 has a broad spectrum of target cells including immature dendritic cells, which express the cognate receptor CCR5.
- Increased expression of CCL4 was reported in the monocytes and blood of elderly individuals. Concentration of CCL4 within the body increases with age, which may cause chronic inflammation and liver damage.
- The assay recognizes mouse CCL4. An antibody specific for mouse CCL4 has been pre-coated onto the microwells.
- The mouse CCL4 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse CCL4 is added to detect the captured mouse CCL4 protein.
- For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm
Specifications
| BC119257 | |
| 5 pg/mL | |
| Sandwich ELISA, Double Antibody | |
| Plasma, Serum | |
| Mouse | |
| Mouse | |
| <10% | |
| Blue ice | |
| 4.5 hr. |
| 15.6 to 1000 pg/mL | |
| ISO 13485 | |
| Coated ELISA Kit | |
| DAB | |
| 20303 | |
| Solid | |
| <10% | |
| -20°C to +4°C | |
| P14097 |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- 96TEST Human IFN-beta ELISA Kit
Signal Word
- Warning
Hazard Category
- Serious eye damage/eye irritation Category 1
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
- Specific target organ toxicity Category 3
Hazard Statement
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H318-Causes serious eye damage.
- H319-Causes serious eye irritation.
- H332-Harmful if inhaled.
- H335-May cause respiratory irritation.
Precautionary Statement
- P201-Obtain special instructions before use.
- P202-Do not handle until all safety precautions have been read and understood.
- P234-Keep only in original container.
- P264-Wash skin thoroughly after handling.
- P270-Do not eat, drink or smoke when using this product.
- P273-Avoid release to the environment.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P308+P313-IF exposed or concerned: Get medical advice/attention.
- P330-Rinse mouth.
- P362-Take off contaminated clothing.
- P405-Store locked up.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : Sulphuric Acid, Tetramethylbenzidine