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Proteintech™ Mouse E-selectin/CD62E ELISA Kit
KE10036 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse E-selectin ELISA kit is to be used to detect and quantify protein levels of endogenous mouse E-selectin.
Brand: Proteintech™ KE10036-96T
This item is not returnable.
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Description
- E-selectin, also known as ELAM-1 or CD62E, is a member of the selectin family of adhesion molecules that also include L-selectin and P-selectin.
- E-selectin is an inducible endothelial cell surface molecule. Its expression on endothelial cells is transcriptionally upregulated by various proinflammatory substances such as IL-1, TNFα and lipopolysaccharide (LPS).
- Besides, it can be secreted by endothelial cells, and can be detected in a soluble form (sE-selectin) in serum. During inflammation, E-selectin plays an important part in recruiting leukocytes to the site of injury.
- The assay recognizes mouse E-selectin. An antibody specific for mouse E-selectin has been pre-coated onto the microwells. The mouse E-selectin protein in samples is captured by the coated antibody after incubation.
- Following extensive washing, another antibody of biotinylated specific for mouse E-selectin is added to detect the captured mouse E-selectin protein.
- For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent.
- Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
Specifications
| NM_011345 | |
| 0.0745 ng/mL | |
| Sandwich ELISA, Double Antibody | |
| Plasma, Serum | |
| Mouse | |
| Mouse | |
| <10% | |
| Blue ice | |
| 4.5 hr. |
| 0.375 to 12 ng/mL | |
| ISO 13485 | |
| Coated ELISA Kit | |
| DAB | |
| 20339 | |
| Solid | |
| <10% | |
| -20°C to +4°C | |
| Q00690 |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- 96TEST Mouse E-selectin/CD62E ELISA Kit
Signal Word
- Warning
Hazard Category
- Serious eye damage/eye irritation Category 1
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
- Specific target organ toxicity Category 3
Hazard Statement
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H318-Causes serious eye damage.
- H319-Causes serious eye irritation.
- H332-Harmful if inhaled.
- H335-May cause respiratory irritation.
Precautionary Statement
- P201-Obtain special instructions before use.
- P202-Do not handle until all safety precautions have been read and understood.
- P234-Keep only in original container.
- P264-Wash skin thoroughly after handling.
- P270-Do not eat, drink or smoke when using this product.
- P273-Avoid release to the environment.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P308+P313-IF exposed or concerned: Get medical advice/attention.
- P330-Rinse mouth.
- P362-Take off contaminated clothing.
- P405-Store locked up.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : Sulphuric Acid, Tetramethylbenzidine
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