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Proteintech™ Mouse IL-17A ELISA Kit

KE10020 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse IL17A ELISA kit is to be used to detect and quantify protein levels of endogenous mouse IL17A.

Brand:  Proteintech™ KE10020-96T


Product Code. 16811616

  • 269.00 € / 96 tests
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Description

Description

  • The assay recognizes mouse IL17A. An antibody specific for mouse IL17A has been pre-coated onto the microwells.
  • The mouse IL17A protein in samples is captured by the coated antibody after incubation.
  • Following extensive washing, another antibody of biotinylated specific for mouse IL17A is added to detect the captured mouse IL17A protein.
  • For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
  • Il17a, also named as Il-17, is a proinflammatory cytokine. Il-17, synthesized only by memory T cells and natural killer cells, has pleiotropic effects, mainly in the recruitment and activation of neutrophils.
  • This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases.
  • This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO).
  • High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.
  • The Il-17 receptor is a type I transmembrane protein, that is widely expressed on epithelial cells, fibroblasts, B and T cells, and monocytic cells. In psoriatic skin lesions, both Th17 cells and their downstream effector molecules, e.g. Il-17 and IL-22, are highly increased.
Specifications

Specifications

NP_034682
2.6 pg/mL
Sandwich ELISA, Double Antibody
Plasma, Serum
Mouse
Mouse
<10%
Blue ice
4.5 hr.
7.8 to 500 pg/mL
ISO 13485
Coated ELISA Kit
DAB
16171
Solid
<10%
-20°C to +4°C
Q62386
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Safety and Handling

Safety and Handling

Product Identifier
  • 96TEST Human IFN-gamma ELISA Kit

Signal Word
  • Warning

Hazard Category
  • Serious eye damage/eye irritation Category 1
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
  • Specific target organ toxicity Category 3

Hazard Statement
  • H302-Harmful if swallowed.
  • H315-Causes skin irritation.
  • H318-Causes serious eye damage.
  • H319-Causes serious eye irritation.
  • H332-Harmful if inhaled.
  • H335-May cause respiratory irritation.

Precautionary Statement
  • P201-Obtain special instructions before use.
  • P202-Do not handle until all safety precautions have been read and understood.
  • P234-Keep only in original container.
  • P264-Wash skin thoroughly after handling.
  • P270-Do not eat, drink or smoke when using this product.
  • P273-Avoid release to the environment.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P308+P313-IF exposed or concerned: Get medical advice/attention.
  • P330-Rinse mouth.
  • P362-Take off contaminated clothing.
  • P405-Store locked up.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information
  • MIXTURE LIST-Contains : Sulphuric Acid, Tetramethylbenzidine

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