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Lonza™ MycoAlert™ Mycoplasma Detection Kit (10 Tests)
Selective biochemical test that exploits the activity of mycoplasmal enzymes that are found in all six of the main mycoplasma cell culture contaminants and the vast majority of 180 mycoplasma species, but are not present in eukaryotic cells.
Brand: Lonza™ LT07-118
This item is not returnable.
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Description
- Convenient and fast.
- Simply add two reagents to the culture supernatant.
- Perform two luminescence readings.
- Results in less than 20 min.
- Easy interpretation of results .
- Detects all common mollicute contaminations (Mycoplasma, Acholeplasma, Entomoplasma and Spiroplasma; except Ureaplasma).
- Negligible interference with media components.
- Selective biochemical test that exploits the activity of mycoplasmal enzymes that are found in all six of the main mycoplasma cell culture contaminants and the vast majority of 180 mycoplasma species, but are not present in eukaryotic cells.
- Viable mycoplasma in a test sample (cell supernatant) are lysed and the enzymes react with the MycoAlert™ Substrate, catalyzing the conversion of ADP to ATP.
- The ATP is then transferred into a light signal via the luciferase enzyme in the MycoAlert™ Reagent.
- By measuring the level of ATP in a sample both before (read A) and after the addition of the MycoAlert™ Substrate (read B), a ratio can be obtained which is indicative of the presence or absence of mycoplasma.
Specifications
| Light yellow | |
| MycoAlert Reagent (lyophilized), MycoAlert Substrate (lyophilized), MycoAlert Assay Buffer | |
| Mycoplasma detection | |
| 2 x 600 μL MycoAlert Reagent, 2 x 600 μL MycoAlert Substrate, 1 x 10 mL MycoAlert Assay Buffer |
Safety and Handling
Hazard Statement
- EUH210-Safety data sheet available on request.
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