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Invitrogen™ No-Stain™ Protein Labeling Reagent
A fast, easy to use, covalent protein labeling reagent, applied to a membrane after gel transfer that provides sensitive, linear detection of protein for total protein normalization of western blotting data. The Invitrogen No-Stain Reagent can also be used as a fast, sensitive gel stain for visualization of proteins in a gel.
Brand: Invitrogen™ A44717
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Description
- Flexible use labeling format—Use with any protein gel of choice to perform total protein labeling of the membrane post-transfer. Or use it as a fast protein stain after electrophoresis of gels you do not intend to transfer.
- Easy to use protocol—Mix and incubate with the transferred membrane. The reaction time is 10 minutes. Gel staining is also a 10 minute reaction.
- Flexible visualization—Use a wide-range of imagers with UV, Green LED or Fluorescence (∼488 mm) light source, including the iBright FL1500 Imaging System, to capture an image.
- Accurate total protein normalization—Designed to provide accurate total protein normalization over a broad linear range for protein detection: 1–80 μg total protein loaded per well.
- Sensitive and stable signal—Protein bands are detected down to 20 ng and the signal is compatible with downstream antibody detection.
- Total protein normalization is a useful method for obtaining accurate, quantitative western blotting data, as housekeeping proteins can often be affected by experimental conditions.
Specifications
| Protein Labeling Reagent | |
| The No-Stain Protein Labeling Reagent forms covalent bonds with the lysine amino acid side chains on all proteins | |
| A standard kit will label 10 mini gels or 10 mini gel-sized membranes | |
| Dry Ice | |
| Mini Precast Protein Gels, Midi Precast Protein Gels | |
| Invitrogen™ |
| • No-Stain activator, 200 μL, store at -5°C to -30°C • No-Stain derivatizer, 200 μL, store at -5°C to -30°C • No-Stain labeling buffer (20X), 2 x 5 mL, store at 15°C to 30°C |
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| Compatible with wide-range of imagers with UV or Fluorescence light sources, for example, the iBright imager | |
| 10 minutes | |
| Fluorescence | |
| PVDF, Nitrocellulose | |
| Compatible with all downstream immunodetection steps (blocking, antibody binding, chemiluminescent or fluorescence based detection |
For Research Use Only. Not for use in diagnostic procedures.