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Invitrogen™ IL-2 Human Matched Antibody Pair

Matched Antibody Pair

Brand:  Invitrogen™ CHC1243

Product Code. 10349254

  • 564.00 € / Each
Estimated Shipment: 28-11-2024
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For Research Use Only. All usage must comply with product instructions.


Includes: Antibody Pair Kit contains matched, pre-titred and fully optimized Capture (coating) and Detection Antibodies, 3 vials of Recombinant Standard, Streptavidin-HRP concentrate and technical data sheet.

Requires : Buffer reagents required to complete reaction available separately (Cat. No. CNB0011).
This item is not returnable. View return policy

Description

Description

The Human IL-2 Antibody Pair Kit comes with pre-matched antibody pairs, standards, and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-2. Buffer reagents needed to complete the ELISA reaction are sold separate as a Buffer Kit for Antibody Pairs (Catalog No. CNB0011). The Assay Buffer included in this Buffer Kit can be used as a blocking reagent for ELISA plates as well as a diluent for ELISA standards and samples, detection antibody, and HRP conjugate. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.

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Specifications

Specifications

P60568
IL2
RUO
Capture (coating) Antibody, Detection Antibody, Standard, Streptavidin-HRP
2°C to 8°C
Colorimetric Microplate Reader
IL-2, TCGF, lymphokine
10 x 96 Tests
3558
Human
ELISA Kit
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For Research Use Only. Not for use in diagnostic procedures.