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Invitrogen™ Recombinant Protein G Agarose

For the purification of IgG from most mammalian species and does not bind IgM, IgA, IgE or serum albumin

Brand: Invitrogen™ 15920010

Product Code. 11598896

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Includes: rProtein G Agarose is supplied as a 50% suspension in PBS (pH 7.0), 20% ethanol.

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Description

Recombinant Protein G (rProtein G) Agarose is a ready-to-use affinity chromatography matrix supplied as a 50% slurry (in phosphate buffered saline (PBS), pH 7.0; 20% ethanol)
The rProtein G is a recombinant form of streptococcal protein G engineered to eliminate nonspecific binding to serum albumin, and is immobilized on 4% agarose beads
rProtein G Agarose has a binding capacity of approximately 20mg of human IgG permL of beads
rProtein G Agarose binds to a broader range of IgG isotypes than react with protein A
The binding characteristics and stability of rProtein G Agarose facilitate rapid and repeated use for affinity chromatography
To purify IgG, load crude samples (serum, ascites fluid, or hybridoma supernatant) onto a column of rProtein G Agarose under physiologic conditions of salt and pH
Non-IgG components do not bind to the beads and elute in the flow through fraction; Purified IgG is then eluted with a low pH buffer

PCR, PCR and Real-Time PCR

Shipping Condition: Wet Ice

Specifications

Content And Storage	rProtein G Agarose is supplied as a 50% suspension in PBS (pH 7.0), 20% ethanol. Store at +4°C.
Separation of	Protein
Shipping Condition	Wet Ice

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For Research Use Only. Not for use in diagnostic procedures.

Description