Invitrogen™ Tau (Total) Human ELISA Kit

Description

The Human Tau (Total) ELISA quantitates Hu Tau in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Tau, independent of phosphorylation state. Principle of the method The Human Tau solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The Tau (Total) Human ELISA Kit is intended for detection of Tau from cerebral spinal fluid (CSF) or from tissue culture supernatant using 96-well plates and a microplate reader.

Easy-to-run sandwich ELISA
A monoclonal capture antibody specific for Hu Tau has been coated onto the wells of the 96-well plate provided. During the first incubation, standards of known Tau content and unknown samples are pipetted into the wells, and the Tau antigen binds to the immobilized (capture) antibody. After washing, a rabbit antibody specific for Tau is added to the wells. During the second incubation, this antibody serves as a detection antibody by binding to the immobilized Tau protein captured during the first incubation. After washing, a horseradish peroxidase-labeled, anti-rabbit IgG is added. This binds to the detection antibody to complete the four-member sandwich. After a third incubation and washing to remove all the unbound enzyme, a substrate solution (TMB) is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of Hu Tau present in the original specimen and the optical density can be read on a standard microplate reader.

Convenient kit format
The kit comes with a capture antibody precoated onto the plate, eliminating an overnight coating process. The plates are machine coated to provide low variability well-to-well. Furthermore, the plate comes as removable 8-well strips, allowing you to run as few samples as you want. Standards provided in the kit can be used to obtain quantitative results from a standard curve or as a positive control.

• Easy to run
• Convenient precoated, removable 8-well strips
• Validated kits for accuracy and consistency
• Each ELISA kit is validated for sensitivity, specificity, precision, and lot-to-lot consistency

See protocol insert for more information on validation.

Cell Analysis, Immunoassays, Ready-To-Use Immunoassay

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