Invitrogen™ Novex™ TBE-Urea Gels, 15%

Description

Novex™ TBE-Urea Gels are denaturing polyacrylamide gels that resolve single-stranded DNA oligos or RNA into sharp, distinct bands. These 15% gels are optimized for the analysis and purification of products ranging from 10–40 bases, making them an ideal choice for synthetic oligo analysis and purification, RNase Protection Assays (RPA), in vitro transcription studies, and northern blot analysis.

Novex TBE-Urea Gels can be stained by silver staining, ethidium bromide, and SYBR™ Green staining techniques after electrophoresis. The compact gels are available in 6%, 10%, or 15% formats and are designed to run on the XCell SureLock™ Mini-Cell.

Advantages of TBE-Urea Gels:
• Contains 7M urea for maximum denaturation
• Excellent resolution for fast size and purity confirmations of DNA or RNA oligos
• High resolution of short single-strand oligonucleotides
• Requires ∼10% sample concentration and volume of large or agarose gels
• Accurate and reproducible results

Formulation
Novex TBE-Urea gels are made with high-purity reagents, including Tris base, boric acid, EDTA, acrylamide, bisacrylamide, TEMED, APS, and 7M urea. Strict quality control ensures that gels and buffers are DNase- and RNase-free.

Recommended Buffers
For optimum performance, Novex™ TBE-Urea sample buffers and Novex™ TBE Running Buffer are strongly recommended for use with these gels. For analytical applications, Novex TBE-Urea Sample Buffer is recommended contains urea, the density agent Ficoll™, which yields sharper, straighter bands than conventional density agents, and the tracking dyes Bromophenol Blue and Xylene Cyanol.

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