Macherey-Nagel™ Nucleospin™ RNA/DNA Buffer Set

Description

With the NucleoSpin™ RNA/DNA buffer set (in combination with the NucleoSpin™ RNA II or NucleoSpin™ RNA Plant kit) a methodology has been developed that enables the isolation of RNA and DNA from undivided samples in one working procedure using one silica-based spin column. DNA and RNA are sequentially eluted from the column using low salt buffer and water, respectively, thus facilitating immediate use of DNA and RNA for common downstream applications. Selective isolation of RNA and DNA from unique, precious samples is a demand for an increasing number of applications. Commonly, two independent preparations – one for RNA and one for DNA – are performed after splitting a sample. For certain samples, however, splitting is unfavourable if not impossible, e.g., biopsy samples or single individuals of small organisms (e.g., insects and worms). Only few methods exist for isolation of RNA and DNA from undivided samples after which RNA and DNA are available separately and immediately ready to use in common downstream applications. Kit components: Buffer set is sufficient for 100 DNA isolations in combination with NucleoSpin™ RNA II or NucleoSpin™ RNA Plant kits, NucleoSpin™ RNA/protein kits.

• Format: Buffer set for elution of DNA in combination with RNA preparations, to be used in combination with NucleoSpin RNA kits
• Sample material: See NucleoSpin RNA II, NucleoSpin RNA XS, NucleoSpin RNA Plant, NucleoSpin RNA protein
• DNA size: 200bp to >30kb
• Typical DNA yield: 5μg from 10⁶ HeLa cells, 16μg from 30mg pig liver, 5μg from 100mg maize leaf
• DNA elution volume: 100μL
  Typical applications:
  For biopsy material: RNA for expression analysis; DNA for mutation analysis, e.g., of cancer genes. For ticks (Ixodes ricinus): RNA for analysis of RNA viruses (eg. TBE-V, Tick-borne encephalitis virus, infectious agent of meningoencephalitis); DNA for analysis of germs (e.g.,Borrelia burgdorferi, infective agent of Lyme disease). For transgenic plants or animals: RNA for expression analysis of the transformed gene and/or other genes; DNA for analysis of integration site, integration number and sequence confirmation of the transformed gene. For transfected culture cells: RNA for expression analysis of the transgene or other genes of interest; DNA for analysis of methylation status of the transgene and other genes of interest.