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Macherey-Nagel™ NucleoZOL
The universal reagent for RNA isolation from cells and tissue, with no chloroform or phase separation.
Brand: Macherey-Nagel™ 740404.200
This item is not returnable.
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Description
NucleoZOL is designed for the isolation of total RNA (small and large RNA) in a single fraction or in separate fractions from a variety of sample materials.
- No chloroform, no phase separation, easy procedure
- High RNA yield and purity from any sample material
- RNA isolation from cultured cells, animal tissue, plant tissue, microorganisms
- Small and large RNA in one or in separated fractions
- RNA ready for use in a variety of downstream applications: RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays
Sample material:
- < 1 x 107 cultured cells, bacteria, and yeast
- < 100 mg human/animal/plant tissue
- < 0.4 mL (viral) fluids
Typical yield:
- Total RNA
- Liver: 6-8 μg/mg tissue
- Kidney, spleen: 3-4 μg/mg tissue
- Muscle, brain, lung: 0.5-1.5 μg/mg
- Cultured cells: 4-10 μg/106 cells
- Large RNA
- Liver: 5-7 μg/mg tissue
- Kidney, spleen: 3-4 μg/mg tissue
- Muscle, brain, lung: 0.5-1.5 μg/mg
- Cultured cells: 3-8 μg/106 cells
Specifications
| 593-84-0; 615-004-00-3 | |
| Room temperature (18 - 25 °C) | |
| Animal/Human/Plant Tissue, Bacteria, Cultured Cells, Viral Fluids, Yeast | |
| Total RNA | |
| RNA isolation from cells and tissue | |
| A260/A280: 1.8 - 2.1 | |
| < 1 hour |
| Blue | |
| One-phase Extraction | |
| Typical RIN: > 9 | |
| Phenol, Guanidinium thiocyanate, Dye | |
| Aromatic | |
| 200 mL | |
| Liquid |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- nucleozol
Signal Word
- Danger
Hazard Category
- Acute toxicity Category 3
- Skin corrosion/irritation Category 1 B
- Germ cell mutagenicity Category 2
- Specific target organ toxicity after repeated exposure Category 2
- LONG-TERM AQUATIC HAZARD Chronic 3
Hazard Statement
- H301-Toxic if swallowed.
- H311-Toxic in contact with skin.
- H314-Causes severe skin burns and eye damage.
- H331-Toxic if inhaled.
- H341-Suspected of causing genetic defects.
- H373-May cause damage to organs.
- H412-Harmful to aquatic life with long lasting effects.
Precautionary Statement
- P201-Obtain special instructions before use.
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P273-Avoid release to the environment.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P405-Store locked up.
Supplemental information
- MIXTURE LIST-Contains : phenol, guanidiniumthiocyanate