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OPTIZYME™ T4 DNA Ligase, Fisher BioReagents™
Brand: Fisher BioReagents BP8099-1
This item is not returnable.
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Description
OPTIZYME™ T4 DNA Ligase catalyzes the formation of phosphodiester bonds between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.
Applications and Features:
- OPTIZYME™ T4 DNA Ligase is used to clone restriction enzyme generated DNA fragments
- Fast sticky end ligation (10min at room temperature)
- Cloning PCR products
- Ligation of double-stranded oligonucleotide linkers or adaptors to DNA
- Site-directed mutagenesis
- Amplified fragment length polymorphism
- Ligase-mediated RNA detection
- Nick repair in duplex DNA, RNA or DNA/RNA hybrids
- Active in buffers for OPTIZYME restriction enzymes
Supplied with:
- 10X OPTIZYME T4 DNA Ligase Buffer : 400mM Tris-HCl, 100mM MgCl2, 100mM DTT, 5mM ATP (pH7.8 at 25°C)
- 50% OPTIZYME PEG 4000 Solution: 50% (w/v) polyethylene glycol
Quality control:
- Endo-, exodeoxyribonuclease, phosphatase and ribonuclease-free
- Functionally tested for ligation of cohesive and blunt end DNA fragments
Specifications
| 5 U/μL | |
| 7.5 | |
| OPTIZYME™ T4 DNA Ligase Buffer 10X: Tris HCl 400mM, MgCl2 100mM, DTT 100mM, ATP 5mM (7.8 pH at 25°C), OPTIZYME™ PEG 4000 Solution 50%: Polyethylene Glycol 50% (w/v) | |
| E. coli cells with a cloned gene 30 of bacteriophage T4 | |
| T4 DNA Ligase |
| Colorless | |
| Used to clone restriction Enzyme generated DNA fragments, cloning PCR products, ligation of double-stranded Oligonucleotide linkers or adaptors to DNA, site-directed mutagenesis, amplified fragment length polymorphism, ligase-mediated RNA detection, nick | |
| -20°C | |
| 100 U | |
| Liquid |
Safety and Handling
Product Identifier
- OPTIZYME