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Tocris Bioscience™ PA Janelia Fluor 646, SE
Red photoactivatable dye (Reactive group: NHS ester). Application: super resolution microscopy (SRM), single molecule tracking and live cell imaging
Brand: Tocris Bioscience™ 6150/300U
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Description
Key information: PA Janelia Fluor™ 646, SE is a photoactivatable fluorescent dye; supplied with an NHS ester reactive group for the labeling of primary amines. Suitable for live and fixed cell imaging. Application: Suitable for single molecule tracking and super resolution microscopy in live cells, specifically sptPALM (live cells) and PALM (fixed cells) techniques. Properties and Photophysical Data: Non-fluorescent until activated at 365 nm. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag™ and SNAP-tag™. Can be multiplexed with PA Janelia Fluor™ 549, SE (Cat. No. 6149) to perform two-color sptPALM in live cells with activation at 405nm. Upon protein conjugation the efficiencies of photoconversion improve substantially. Low background staining. Excitation and emission maxima (λ) are 649 - 651 nm and 663 - 665 nm, respectively. Please see the protocol for further information on protein/antibody labeling and conjugation. HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.Specifications
| 2,5-Dioxo-1-pyrrolidinyl 3,7-di-1-azetidinyl-2-diazo-5,5-dimethyl-2,3-dihydro-5H-3-oxospiro[dibenzo[b,e]siline-10,1-indene]-6-carboxylate | |
| 300 μg | |
| C34H31N5O5Si |
| 2093120-32-0 | |
| Photoactivatable Dyes | |
| 90% |
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