IBA Lifesciences pASK-IBA E. coli vector

pASK-IBA vectors are classic cloning vectors devised for periplasmic or cytoplasmic E. coli expression of recombinant proteins with the Strep-tag™II fused to the C- or N-terminus. They carry the chloramphenicol resistance cassette for selection of transformed E. coli cells, F1 and ColE1 origin for a high plasmid copy number, a multiple cloning site with recognition sites for several common restriction enzymes (e.g. EcoRI, SmaI, BamHI, SalI, PstI) and the inducible tetracycline promoter/operator for the regulated expression of proteins. Some carry the ompA signal for periplasmic secretion of the recombinant protein.The vector can be combined with any E. coli strain since the tet-promoter works independently of the genetic background of E. coli. Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity. During secretion into the periplasmic space the ompA signal sequence will be cleaved off.