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Invitrogen™ Phospha-Light™ SEAP Reporter Gene Assay System
Chemiluminescent reporter gene assay for sensitive detection of secreted human placental alkaline phosphatase used as a reporter protein.
Brand: Invitrogen™ T1015
376.20 EUR valid until 2024-09-17
Use promo code "21690" to get your promotional price.
Additional Details : Weight : 0.01000kg
Includes: Substrate, required buffers, enhancer and control Placental Alkaline Phosphatase
Description
- Detection of secreted reporter permits analysis without destroying valuable cells
- Wide dynamic range of alkaline phosphatase assay lets user measure enzyme level accurately from femtogram to nanogram range
- Assay sensitivity is 100- to 1,000-fold better than with either the isotopic/non-isotopic assays for chloramphenicol acetyl transferase (CAT) or the colorimetric/fluorescent assays for b-galactosidase, providing greater sensitivity than competing assays
- Highly sensitive assay with a wide dynamic range permits detection of high and low levels of reporter without the need to perform numerous sample dilutions
- Non-radioactive reporter gene assay kit eliminates concerns over use of radioisotopes
- Assay can be completed in about one hour, providing fast assay turnaround
- Compatible Cells: Mammalian Cells
- Detection Method: Chemiluminescent
- For Use With Equipment: Luminometer (Microplate)
- Form: Liquid
- Format: 96-well plate
- High Throughput Compatibility: High Throughput-Compatible
- Readout: End Point
- Sample Type (Specific): Cell Cultures
- Substrate: CSPD™
- Substrate Properties: Chemical Substrate
- Substrate Type: AP (Alkaline Phosphatase) Substrate
- Target Enzyme: Alkaline Phosphatase
Specifications
Secreted Human Placental Alkaline Phosphatase Reporter Gene Assay System | |
Luminometer (Microplate) | |
This kit contains the substrate, required buffers, enhancer, and control Placental Alkaline Phosphatase. Store the product at 4°C. |
|
High-throughput Compatible | |
End Point | |
CSPD™ | |
Chemical Substrate | |
NovaBright™, Phospha-Light™ | |
400 Assays |
Chemiluminescent | |
Reporter Assay | |
Mammalian Cells | |
Unconjugated | |
Cell Cultures | |
AP (Alkaline Phosphatase) Substrate | |
Alkaline Phosphatase | |
Enzyme Assay | |
Liquid |
Safety and Handling
- Phospha-Light SEAP Reporter Gene Assay System
Signal Word
- Danger
Hazard Category
- Acute toxicity Category 4
- Serious eye damage/eye irritation Category 1
- Skin corrosion/irritation Category 2
- Specific target organ toxicity after repeated exposure Category 2
Hazard Statement
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H318-Causes serious eye damage.
- H373-May cause damage to organs.
Precautionary Statement
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P264-Wash hands thoroughly after handling.
- P270-Do not eat, drink or smoke when using this product.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P310-Immediately call a POISON CENTER/doctor/.
- P314-Get medical advice/attention if you feel unwell.
- P330-Rinse mouth.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- P362+P364-Take off contaminated clothing and wash it before reuse.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : Diethanolamine
For Research Use Only. Not for use in diagnostic procedures.