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Invitrogen™ Phosphate Sensor
Phosphate Sensor
Brand: Invitrogen™ PV4406
This item is not returnable.
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Description
The Phosphate Sensor is a highly sensitive reagent for the detection of inorganic phosphate, either in real-time kinetic or end-point mode. This product is a purified form of recombinant E. coli phosphate-binding protein labeled with the fluorophore MDCC, which is sensitive to changes in its environment. Binding of inorganic phosphate by Phosphate Sensor is rapid and tight (Kd ∼ 0.1μM), resulting in a large increase in fluorescence. The Phosphate Sensor detects phosphate in the high-nanomolar to low-micromolar range, which is 100-fold more sensitive than malachite green. This reagent provides you with a sensitive assay for detecting enzymes that produce inorganic phosphate either directly or indirectly through coupled reactions, such as phosphatases, ATPases, GTPases, and phosphodiesterases.![TRUSTED_SUSTAINABILITY](/content/dam/fishersci/glyphs/Sustain_Badge.png)
Specifications
Microplate Reader | |
Fluorescence Intensity | |
Phosphate Sensor is supplied as a 500 μM solution in 10 mM Tris (pH 7.6) and 50 mM NaCl. Store product at -80°C. | |
MDCC | |
10 nmol | |
430⁄450 | |
Dry Ice |
GTPases, ATPases, Phosphatases, Phosphodiesterases | |
Phosphatase Assay | |
Fluorescence | |
Phosphate Sensor | |
384-well plate | |
Kinetic |
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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