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Thermo Scientific™ Zeba™ Dye and Biotin Removal Spin Columns and Filter Plates, 0.5–10 mL

Enable fast and efficient removal of non-reacted fluorescent dyes, biotin, reducing agents, and crosslinkers from protein samples with Thermo Scientific Pierce Zeba Dye and Biotin Removal columns and filter plates.

Brand:  Thermo Scientific™ A44302

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Product Code. 16607425

  • 149.00 € / Each
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For Research Use Only. All usage must comply with product instructions.

This item is not returnable. View return policy

Description

Description

Quickly remove non-reacted fluorescent dyes, biotin, reducing agents, and crosslinkers from protein samples with a 7K MWCO in fewer than 15 minutes with Thermo Scientific Pierce Zeba Dye and Biotin Removal columns and filter plates.

Removing free-dye after a labeling reaction is often difficult and time-consuming but is essential for accurate determination of dye-to-protein ratios. Pierce Zeba Dye and Biotin Removal resin is highly specialized to produce exceptional protein recoveries while effectively removing non-conjugated fluorescent dyes, biotinylation reagents, reducing agents, and crosslinkers. When using the appropriate amount of sample and working within buffer conditions, these columns and filter plates can successfully remove almost any fluorescent dye. Even dilute protein samples (25 μg/mL) can be successfully processed to remove dyes, biotin, reducing agents, and crosslinkers and to efficiently recover proteins (>7 kDa). These products are not recommended for desalting or buffer exchange.

Features of Zeba Dye and Biotin Removal columns and filter plates include:
Versatile—removes unreacted fluorescent dyes, biotinylation reagents, crosslinkers, and reducing agents from protein solutions
High recovery—low-binding resin maximizes protein recovery
Easy-to-use—no cumbersome column preparation or equilibration
Fast—small-molecule removal and protein recovery in less than 15 minutes
Flexible—available in spin columns and filter spin plates for a range of needs

Sample capacities
• 0.5-mL spin column: 50 to 120 μL sample
• 2-mL spin column: 400 to 700 μL sample
• 5-mL spin column: 1 to 2 mL sample
• 96-well filter plate: 40 to 100 μL sample (per well)

Available formats
Spin columns: 0.5, 2, 5, and 10 mL
96-well filter plates: pre-dispensed; compatible with centrifugation for manual or automated purification; enable fast, consistent well-to-well and plate-to-plate reproducibility for small-scale, high-throughput clean-ups

Applications
• Ideal for post-reaction clean-up of samples
• To effectively remove non-conjugated dye, biotin, reducing agents, and crosslinkers with exceptional protein recovery

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Store in refrigerator (2–8°C).
Spin Column
Zeba™
10 mL
Room Temperature
Proprietary Resin
5 Columns
2 to 4 mL
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