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  5. Thermo Scientific™ Pierce™ ECL Plus Western Blotting Substrate
  6. 11527271

Thermo Scientific™ Pierce™ ECL Plus Western Blotting Substrate

Acridan-based chemiluminescent and chemifluorescent HRP substrate for Western blot detection using X-ray film or CCD- or laser-based imagers.

Brand:  Thermo Scientific™ 32132X3

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Regulatory: This product contains a substance listed in Annex XVII to REACH Regulation (EC) No 1907/2006, all customers must complete a declaration before delivery.

Product Code. 11527271

  • 780.00 € / Pack of 3

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Description

Description

Thermo Scientific™ Pierce ECL Plus Substrate is an acridan-based chemiluminescent and chemifluorescent substrate to detect peroxidase activity from HRP-conjugated probes by Western blotting using X-ray film, CCD or laser-based imagers.

Thermo Scientific Pierce ECL Plus Substrate is an acridan-based chemiluminescent and chemifluorescent HRP substrate for western blot detection using X-ray film or CCD- or laser-based imagers. This product is sold as Pierce ECL 2 Substrate (Cat. #PI80196X3) through Fisher Scientific and other channels.

Features of ECL Plus Substrate:

  • Easy to use—can be substituted for the discontinued GE ECL Plus Substrate without any re-optimization
  • Higher sensitivity—detect targets down to the low-picogram level
  • Longer signal duration—sustained light output for as long as 5 hours
  • More imaging options—X-ray, CCD or laser-based imagers
  • More affordable—high quality and performance at a lower price than other suppliers' similar substrates

    Pierce ECL Plus Substrate generates acridinium esters when it reacts with HRP. As these ester intermediates react with peroxide, they produce a strong and sustained chemiluminescent signal that can be captured by X-ray film CCD imaging. The substrate also produces a robust fluorescent signal that can be captured with fluorescence imagers. Pierce ECL Plus Substrate enables the detection of low-picogram amounts of target protein on nitrocellulose or PVDF membrane when probed with appropriate primary and secondary antibody concentrations. Its broad dynamic range, high sensitivity and long-lasting signal make Pierce ECL Plus Substrate an excellent choice for western blot analysis.
    TRUSTED_SUSTAINABILITY
    • Specifications

    Specifications

    Substrate
    Liquid
    Nitrocellulose, PVDF
    300 mL Kit
    3000 sq. cm of membrane
    Chemiluminescence, Fluorescence
    Western Blot
    Pierce™
    Low picogram to mid-femtogram
    Upon receipt store at 4°C. Product shipped on ice.
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    For Research Use Only. Not for use in diagnostic procedures.