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Thermo Scientific™ Pierce™ Glycoprotein Staining Kit
Rapidly stain glycosylated proteins in polyacrylamide gels using the periodic acid-Schiff (PAS) method in this convenient kit.
Brand: Thermo Scientific™ 24562
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Description
Thermo Scientific™ Pierce Glycoprotein Staining Kit is a convenient, fast and sensitive kit for staining glycosylated proteins in polyacrylamide gels using the periodic acid-Schiff (PAS) method.
The kit provides three essential reagents and a complete protocol to specifically stain glycosylated proteins (glycoproteins) that have been separated by polyacrylamide gel electrophoresis (PAGE). A gel or membrane containing separated proteins is treated with a periodate solution, which oxidizes cis-diol sugar groups in glycoproteins. The resulting aldehyde groups are detected through the formation of Schiff-base bonds with a reagent that produces magenta bands. Traditionally called the periodic acid-Schiff (PAS) reagent method, this staining technique is made easier by the convenient packaging of essential reagents in one kit with easy-to-follow instructions. The kit includes positive and negative control proteins.Highlights:
Specific for glycoproteins – periodic acid-Schiff (PAS) method specifically detects glycosylated proteins having sialic acid and other oxidizable carbohydrate groups
Optimized protocol – the traditional three-reagent method has been streamlined to achieve optimal staining in less than two hours (half the time required for other staining methods)
Colorimetric – glycoproteins are detected as magenta bands against a faint pink or colorless gel background
Qualitative staining – detection limit depends on the extent of glycosylation and protein size and abundance; detects 0.625ng avidin and 0.16g horseradish peroxidase per band
Controls included – complete kit includes positive and negative control proteins (HRP and STI, respectively).
Gels and membranes – effective for use with polyacrylamide mini gels or nitrocellulose membranes
Includes:
Three reagents in 250mL bottles (1 solution and 2 dry powders to which water must be added), plus two vials of lyophilized proteins (1mg each)
Specifications
| Sufficient For: 10 mini gels (SDS-PAGE) • Oxidation Reagent, 2.5 g powder to make 250 mL • Reduction Reagent, 1.25 g powder to make 250 mL • Positive Control (Horseradish Peroxidase), 1 mg • Negative Control (Soybean Trypsin Inhibitor), 1 mg • Glycoprotein Stain, 250 mL Store all components at 4°C. |
|
| Colorimetric | |
| Glycoprotein Staining Kit | |
| 750 mL |
| In-Blot Detection, In-Gel Detection | |
| Fuchsin Sulfite | |
| Pierce™ | |
| Proteins (Glycoproteins) |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
- Pierce Glycoprotein Staining Kit
Signal Word
- Danger
Hazard Category
- Oxidising solid Category 2
- Acute toxicity Category 4
- Skin corrosion/irritation Category 1 B
- Serious eye damage/eye irritation Category 1
- Skin sensitiser Category 1
- Respiratory sensitiser Category 1
- Carcinogenicity Category 1B
Hazard Statement
- H272-May intensify fire; oxidiser.
- H302-Harmful if swallowed.
- H314-Causes severe skin burns and eye damage.
- H317-May cause an allergic skin reaction.
- H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
- H350-May cause cancer.
- EUH031-Contact with acids liberates toxic gas.
Precautionary Statement
- P201-Obtain special instructions before use.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P284-¢In case of inadequate ventilation! wear respiratory protection.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P220-Keep/Store away from clothing/combustible materials.
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P264-Wash thoroughly after handling.
- P270-Do not eat, drink or smoke when using this product.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P331-Do NOT induce vomiting.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P305-IF IN EYES:.
- P310-Immediately call a POISON CENTER/doctor/.
- P501-Dispose of contents/container to .
For Research Use Only. Not for use in diagnostic procedures.