Thermo Scientific™ Pierce™ Protein G Magnetic Beads

Description

Perform antibody purification and immunoprecipitation methods using manual or robotic magnetic separators with Thermo Scientific™ Pierce Protein G Magnetic Beads, high-capacity and high-throughput affinity particles.

Additional Information and Online Ordering

Recombinant protein G, covalently attached to a blocked magnetic bead surface, can bind to antibodies from many different species, enabling purification of antibodies from crude extracts. Immunoprecipitation assays performed with protein G coated beads result in high yield of target antigen with very low background. Using our crosslinker chemistry, you can immobilize an antibody onto the magnetic particle and prevent IgG contamination in IP or Co–IP experiments. The Pierce Protein G Magnetic Beads can be used both manually with a magnetic stand as well as with automated platforms such as the Thermo Scientific KingFisher Instruments for high-throughput workflows.

Highlights:

• High efficiency – equivalent or higher yield of IP target antigens than magnetic beads from other suppliers

• Low non-specific binding – stable, pre–blocked beads provide highly purified product (e.g., antigen eluted in IP with antibody is devoid of contaminating protein from complex cell lysate)

• Consistent – magnetic beads eliminate resin loss and provide for more efficient separation of solutions than traditional IP methods that use only centrifugation

• Versatile – beads are compatible with manual and automated workflows (e.g., Thermo Scientific™ KingFisher Instruments)

Recommended for:

• IP and Co-IP experiments
• Antibody purification