Detect mouse and human antibodies, especially IgG isotypes, in immunoassays with this purified, biotinylated or peroxidase-conjugated recombinant Protein G.
Brand: Thermo Scientific™ 31499
Purified and conjugated forms of Thermo Scientific™ Pierce Recombinant Protein G enable probing and detection of mouse and human antibodies, especially IgG isotypes, in Western blotting, ELISA, IHC and other immunoassay protocols.
Protein G is a bacterial cell wall protein isolated from group G Streptococci. DNA sequencing of native Protein G identifies two IgG-binding domains and sites for albumin and cell surface binding. The albumin and cell surface binding domains have been eliminated from Recombinant Protein G to reduce nonspecific binding and, therefore, can be used to separate IgG from crude samples. Optimal binding occurs at pH 5, although binding is also effective at pH 7.0 to 7.2.Highlights:
| Protein G, Peroxidase Conjugate | |
| 0.5 mg | |
| Powder | |
| Antibodies |
| HRP | |
| Pierce™ | |
| Protein G | |
| Upon receipt store lyophilized product at -20°C. Product is shipped at ambient temperature. |
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For Research Use Only. Not for use in diagnostic procedures.
