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Thermo Scientific™ Yeast beta-Galactosidase Assay Kit
Extract protein from yeast two-hybrid reporter systems and measure beta-galactosidase activity using compatible Y-PER Reagent and b-Gal Assay Reagent.
Brand: Thermo Scientific™ 75768
This item is not returnable.
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Description
Thermo Scientific™ Yeast β-Galactosidase Assay Kit is specially formulated for yeast cells and ideal for identifying protein interactions in vivo using two-hybrid systems.
The Yeast β-Galactosidase Assay Kit allows for qualitative or quantitative determination of β-galactosidase activity in solution directly from colonies growing on solid medium. A portion of the colony is suspended in a mixture of Thermo Scientific Y-PER™ Yeast Protein Extraction Reagent and the β-Galactosidase Assay Buffer. After a brief incubation period, the solution turns yellow from the hydrolysis of o-nitrophenyl-β-D-galactopyranoside (ONPG) to o-nitrophenol (ONP) and galactose in a mildly alkaline solution. The assay becomes quantitative if calibrated to cell number by measuring the optical density at 660nm.Highlights:
- Efficient lysis of yeast cells and a colorimetric detection system
- Quantitative or qualitative assay for yeast colonies grown on solid media or in suspension
- Colorimetric assay easily measured in a plate reader or spectrophotometer (420nm)
- Adaptable to 96-well microplates or tube assay formats
- Perform homogeneous screening assays or measure activity secondarily after cell lysis
- Also compatible with prepared lysates from other species such as bacteria
Includes:
Thermo Scientific Y-PER Yeast Protein Extraction Reagent (Part No. 78990), 2X Assay Buffer and Stop Solution
Specifications
| Sufficient For: 250 colonies or 250 culture samples (100 μL each) |
|
| Absorbance | |
| Yeast beta-Galactosidase Assay Kit | |
| 96-well Plate | |
| 75 mL | |
| Beta-Gal Substrate | |
| Beta-Galactosidase |
| Reporter Enzyme | |
| Microplate Reader | |
| Enzyme Labeled | |
| Pierce™ | |
| ONPG | |
| 250 colonies or 250 culture samples (100 μL each) |
For Research Use Only. Not for use in diagnostic procedures.