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pJTI™ R4 EXP CMV-TO EmGFP pA Vector
pJTI™ R4 EXP CMV-TO EmGFP pA Vector
Brand: Invitrogen A15005
This item is not returnable.
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Description
The pJTI™ R4 Exp CMV-TO EmGFP pA vector is a positive control vector for assessing retargeting efficiency when retargeting a Jump-In™ T-REx™ parental cell line. When co-transfected with the integrase vector (pJTI™ R4 Int vector included in the Jump-In™ parental kits) and after antibiotic selection, the EmGFP can be inducibly expressed with doxycycline and the successfully retargeted cells will fluoresce green.Ensure the Success of Your Jump-In™ T-REx™ Retargeting Reactions
Successful retargeting of Jump-In™ T-REx™ parental cell lines like the Jump-In™ T-REx™ HEK293 Kit is dependent on a variety of factors, such as:
We strongly recommend including a positive control retargeting reaction using the pJTI™ R4 Exp CMV-TO EmGFP pA vector in your Jump-In™ experiment, along with negative controls (no plasmid DNA, no integrase vector), so you can easily visualize the results and optimize the retargeting conditions.
Specifications
| Inducible | |
| Tetracycline | |
| Mammalian Expression Vector | |
| Geneticin™ (G-418) | |
| Ampicillin (AmpR) | |
| 100 μg | |
| Jump-In™ |
| Transfection | |
| CMV/TO | |
| GFP (EmGFP) | |
| pJTI™ R4 EXP CMV-TO EmGFP pA vector (100 μg), store at -20 °C | |
| GFP (EmGFP) | |
| Jump-In Vectors | |
| Stable Cell Line Development, Targeted Integration, Regulated Expression |
For Research Use Only. Not for use in diagnostic procedures.