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Molecular Probes™ Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit

Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit

Brand: Molecular Probes™ A24881

Product Code. 15554384

806.00 € / Pack of 40

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For Research Use Only. All usage must comply with product instructions.

This item is not returnable. View return policy

Description

The Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit enables optimal image-based analysis of four key markers of human pluripotent stem cells (PSCs): OCT4, SOX2, SSEA4, and TRA-1-60. This high performance immunocytochemistry (ICC) kit includes a complete set of primary and secondary antibodies, a nuclear DNA stain, and premade buffers for an optimized staining experiment. To characterize pluripotent stem cells using only two markers, we recommend us of the Pluripotent Stem Cell Immunocytochemistry Kit (OCT4, SSEA4) or the Pluripotent Stem Cell Immunocytochemistry Kit (SOX2, TRA-1-60).

With the Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit, you can:

Confirm expression of key pluripotent stem cell markers with highly specific antibodies

Get more information per sample by measuring more than one marker at a time

Generate beautiful multiplexed images with minimal effort

Save time and avoid cell loss by minimizing unnecessary wash steps in your staining protocol

Process samples with confidence using a complete and optimized set of ICC reagents

Confirm Expression of Key PSC Markers
The antibodies included in the kit have been carefully selected and validated for high performance in the ICC analysis of PSCs, whether cultured under feeder-dependent or feeder-free media systems. OCT4 and SOX2 are critical nuclear markers, while SSEA4 and TRA-1-60 are important cell surface markers.

Get More Information Per Sample
The antibody combinations in the kit have been designed to allow for the specific and simultaneous assessment of two markers at a time (e.g., OCT4 & SSEA4 and SOX2 & TRA-1-60) along with nuclear DNA staining to help save time and precious sample.

Generate Beautiful Multiplexed Images
Powered by the superior performance of bright, photostable Alexa Fluor™ dyes, this kit enables the acquisition of beautiful multiplexed images of PSC marker expression using commonly available blue, green, and orange/red filter sets.

Minimize Unnecessary Wash Steps
The streamlined method recommended for this kit eliminates unnecessary wash steps found in standard ICC protocols to help save time and decrease the likelihood of cell loss during the staining protocol, especially when using glass coverslips or chamber slides. The only washes required are after incubation with primary and secondary antibodies.

Process Samples with Confidence
An optimized set of premade fixation, permeabilization, blocking, and wash buffers allows you to focus on answering scientific questions rather than spending time preparing reagents and determining compatibility.

What You Get
Easily scaled to any culture plate, dish, or slide format, the Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit comes with reagents sufficient to stain 40 samples using a 50 uL staining volume. (See kit content details below.)

Specifications

Conjugate	2 Color, Alexa Fluor 488, Alexa Fluor 555, Alexa Fluor 594
Quantity	40 tests
Content And Storage	Store at -20°C to 4°C: 10 μL of Rabbit Anti-Human OCT4 (200X) 20 μL of Rat Anti-Human SOX2 (100X) 20 μL of Mouse Anti-Human SSEA4 (100X) 20 μL of Mouse Anti-Human TRA-1-60 (100X) 20 μL of Alexa Fluor™ 555 Donkey Anti-Rabbit (250X) 20 μL of Alexa Fluor™ 594 Donkey Anti-Rabbit (250X) 20 μL of Alexa Fluor™ 488 Donkey Anti-Rat (250X) 20 μL of Alexa Fluor™ 555 Goat Anti-Mouse IgM (250X) 20 μL of Alexa Fluor™ 594 Goat Anti-Mouse IgM (250X) 20 μL of Alexa Fluor™ 488 Goat Anti-Mouse IgG3 (250X) Store at -20°C to RT: 1 vial of NucBlue™ Fixed Cell ReadyProbes™ Reagent (1X) 10 mL of Fixative Solution (1X) 10 mL of Permeabilization Solution S (1X) 20 mL of Blocking Solution (1X) 20 mL of Wash Buffer (10X)
Product Type	Immunocytochemistry Kit

Product Suggestions Safety and Handling

Safety and Handling

Product Identifier

Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit

Signal Word

Danger

Hazard Category

Carcinogenicity Category 1B
Serious eye damage/eye irritation Category 1
Germ cell mutagenicity Category 2
Skin sensitiser Category 1
Specific target organ toxicity Category 2

Hazard Statement

H317-May cause an allergic skin reaction.
H318-Causes serious eye damage.
H341-Suspected of causing genetic defects.
H350-May cause cancer.
H371-May cause damage to organs.

Precautionary Statement

P201-Obtain special instructions before use.
P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
P264-Wash hands thoroughly after handling.
P270-Do not eat, drink or smoke when using this product.
P280-Wear protective gloves/protective clothing/eye protection/face protection.
P302+P352-IF ON SKIN: Wash with plenty of water/soap.
P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P308+P313-IF exposed or concerned: Get medical advice/attention.
P310-Immediately call a POISON CENTER/doctor/.
P332+P313-If skin irritation occurs: Get medical advice/attention.
P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

MIXTURE LIST-Contains : Paraformaldehyde

Documents

Product Information

Manuals

User Guide: PSC Immunocytochemistry Kits Version: D.0 File size: 328.4 kB

Certificates

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Lot Number

5 results found

Lot Number	Certificate Type	Date	Catalog Number
Lot Number3190321	Certificate TypeCertificate of Analysis	Date28/03/2025	Catalog NumberA24881
Lot Number3190323	Certificate TypeCertificate of Analysis	Date24/03/2025	Catalog NumberA24881
Lot Number3190321 (1)	Certificate TypeCertificate of Analysis	Date24/03/2025	Catalog NumberA24881
Lot Number3109388	Certificate TypeCertificate of Analysis	Date21/02/2025	Catalog NumberA24881
Lot Number3166988	Certificate TypeCertificate of Analysis	Date21/02/2025	Catalog NumberA24881

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