Invitrogen™ ProLong™ Gold Antifade Mountant with DNA Stain DAPI

Description

ProLong Gold Antifade Mountant with DNA Stain DAPI is a mounting media that can be directly applied to fluorescently labeled cell or tissue samples on microscope slides. It comes ready-to-use and stored at room temperature—just apply a drop, add a coverslip, cure, and image. ProLong Gold Antifade Mountants come in a 2-mL dropper bottle or 10-mL bottle. It is formulated with the blue DNA stain DAPI to make your nuclear counterstaining more convenient and consistent when preparing your samples for imaging.

ProLong Gold Antifade Mountant with DNA Stain DAPI contains chemical components designed to protect fluorescent dyes from fading (photobleaching) during fluorescence microscopy experiments. It is a curing mounting media that forms an optical path of 1.47 RI and allows longer-term storage of the sample.

Many mountants can quench initial fluorescence signal strength by up to 80%. ProLong Gold Antifade Mountant was developed to minimize this initial signal quenching for the broadest range of colors and dyes and is formulated with the DAPI DNA nuclear stain to simplify your experiments. DAPI stain is excited by UV light at 360 nm when bound to DNA, with an emission maximum at 460 nm, and is detected using a DAPI traditional filter.

ProLong Gold Antifade Mountant is not recommended for mounting samples containing fluorescent proteins such as GFP or TagRFP but can be used with Alexa Fluor dyes of your choice. For superior antifade protection of fluorescent proteins and fluorescent dyes, ProLong Diamond or ProLong Glass Antifade Mountants are recommended. ProLong Glass mountants are recommended for all immersion oil imaging applications. For immediate viewing of a sample, choose our non-curing mountant, SlowFade Gold Antifade Mountant with DAPI.