missing translation for 'onlineSavingsMsg'
Learn More

Thermo Scientific™ Rapid Equilibrium Dialysis (RED) Inserts and Plates

Equilibrium dialysis is a method used in plasma protein binding (PPB) studies to determine the amount of free, active drugs present in plasma. The Rapid Equilibrium Dialysis (RED) Device is widely accepted as the gold standard device for measuring interactions between drugs and PPB.

Brand:  Thermo Scientific™ 89812

134.30 EUR valid until 2024-12-20
Use promo code "21651" to get your promotional price.


 View more versions of this product

Product Code. 10568913

  • 158.00 € / Each

Please to purchase this item. Need a web account? Register with us today!

Explore more special offers
This item is not returnable. View return policy

Description

Description

Rapid Equilibrium Dialysis (RED) Devices have been widely adopted as an effective in vitro technique for conducting plasma protein binding (PPB) assays in drug development. This measurement is used to assess the biological availability of drug candidates and guides critical downstream decisions such as drug design, in vivo testing prioritization, and drug-drug interaction evaluation. RED Devices were developed through close collaboration with the pharmaceutical industry, ensuring that they meet the highest standards of accuracy and reliability in equilibrium dialysis testing.

Equilibrium dialysis is commonly used in drug-plasma protein binding (PPB) studies to quantitate the free fraction of drugs in plasma. The binding of drugs to plasma proteins is a critical factor that affects their pharmacokinetics and pharmacodynamics, as only the free fraction of a drug can exert a physiological effect. The Thermo Scientific Pierce Rapid Equilibrium Dialysis (RED) Device is a superior method for conducting this analysis because it delivers accurate and consistent data with shorter preparation and dialysis times.

RED Devices use a size-defined dialysis membrane to quantitate the amount of free (active) drug molecules that are not bound to plasma proteins. They consist of disposable inserts and a base plate with a standard microplate footprint. The inserts are made of two side-by-side chambers separated by a dialysis membrane and are used along with a required base plate or are available as single-use plates with Inserts. Competition RED Devices are also available to enable competitive dialysis experiments with multiple tissue or protein fluid samples.

Features of RED Devices include:
Ease of use—disposable tubes do not require presoaking, assembly, or specialized equipment
Rapid dialysis—high surface-to-volume ratio of the membrane enables equilibrium in 2–4 hours
High-throughput—96-well footprint is suitable for automation
Multi-sample processing—can be used for 1–48 assays/plate without wasting an entire plate
Robustness—compartmentalized design eliminates the potential for cross-contamination or leakage
Reproducibility and accuracy—validated for plasma protein binding assays, producing results consistent with those reported in the literature
Flexible format—available in pre-inserted disposable polypropylene plates and packs of individual inserts

In addition to plasma protein binding, the device is used for determining drug partitioning between red blood cells and plasma, protein binding of liver microsomes to improve the correlation between in vitro and in vivo intrinsic clearance, drug metabolism and pharmacokinetics (DMPK) assays, and competition between tissue protein binding against plasma proteins.

RED Device Inserts, 8K MWCO
Pierce RED Device Inserts are designed for use with a required base plate for equilibrium dialysis experiments. Each single-use, disposable insert is made of two side-by-side chambers separated by an O-ring-sealed vertical cylinder of dialysis membrane (8K MWCO) validated for minimal nonspecific binding. They are available in packs of 50 or 250 each and are useful for testing a variable number of samples per run.

RED Device Base Plates
Pierce RED Device Inserts are used with either the RED Device Reusable Base Plate made of high-grade PTFE (Teflon™) or the RED Device Single-Use Base Plate made of high-density polypropylene. The Single-Use Base Plate is disposable and lightweight, allowing routine automation. Each Base Plate holds up to 48 RED Device Inserts and has a standard 96-well plate footprint with 9 x 9 mm well spacing for compatibility with multichannel pipettors and automated liquid handling systems.

RED Device Single-Use Plate with Inserts, 8K or 12K MWCO
The RED Device Single-Use Plate with Inserts is composed of disposable high-density polypropylene and comes preloaded with 48 equilibrium dialysis membrane inserts. Each insert includes two side-by-side chambers separated by an O ring sealed vertical cylinder of dialysis membrane (8K or 12K MWCO). The device is automation-friendly and has a standard 96-well plate footprint with 9 x 9 mm well spacing. The single-use plate is easily disposed of to avoid contamination and cleaning, making it useful when performing PPB assays with radioactive or hazardous materials.

Competition RED Device Inserts and Base Plate, 12K MWCO
The Pierce Competition RED Device facilitates the analysis of simultaneous drug interactions and partitioning among multiple tissues to accurately model in vivo drug interactions. The system consists of disposable dialysis tube inserts (12K MWCO) and a reusable base plate made of chemically inert high-grade PTFE, eliminating non-specific binding and risk of contamination. The base plate is divided into different size chambers for positioning 2–8 RED Device Inserts per well, enabling competitive dialysis experiments with 2–15 separate tissue or protein fluid samples. Each Competition RED Insert contains either one or two separate dialysis chambers (each package includes both types).

Applications of the Competition RED Devices include:
• ADME-Tox studies: in vitro screening of drug partitioning between plasma and multiple tissues before in vivo studies with animal models
• Determine formulation of drug dosage for in vivo studies
• Drug-drug interaction studies
• Competitive binding and dissociation constant determination for small molecules versus multiple targets

RED Device Insert Removal Tool
This tool enables fast removal of 8 inserts at once.

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Regenerated Cellulose, Dialysis
Equilibrium Dialysis
Pierce
1 Tool
RED Device Insert Removal Tool
Product Suggestions

Product Suggestions

Videos
SDS
Documents

Documents

Certificates
Promotions

Promotions

Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.

Patent pending.