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Invitrogen™ ReadyProbes™ Tissue Autofluorescence Quenching Kit

Minimizes unwanted autofluorescence in FFPE and frozen tissues due to aldehyde fixation, red blood cells, and structural elements such as collagen and elastin

Brand:  Invitrogen™ R37630

129.96 EUR valid until 2024-12-31
Use promo code "21651" to get your promotional price.

Product Code. 17162534

  • 171.00 € / 1 set
Estimated Shipment: 10-01-2025
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For Research Use Only. All usage must comply with product instructions.

This item is not returnable. View return policy

Description

Description

  • The ReadyProbes Tissue Autofluorescence Quenching Kit minimizes unwanted autofluorescence in FFPE and frozen tissues due to aldehyde fixation, red blood cells, and structural elements such as collagen and elastin.
  • If the target signal is low enough, then this background signal can interfere with the interpretation of immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence(IF), and in situ hybridization (ISH) assays.
  • The ReadyProbes Tissue Autofluorescence Quenching Kit minimizes most kinds of autofluorescence, with the exception of autofluorescence from lipofuschin granules.
  • The ReadyProbes Tissue Autofluorescence Quenching Kit contains three components that are combined just before use. Combine components A, B, and C in a 1:1:1 ratio.
  • Mix components A and B together before adding component C. The resulting solution is then applied to the tissue for 2–5 minutes at room temperature.
  • This is done after the blocking, primary antibody, and secondary antibody steps, but before application of any counterstains like nuclear stains.

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Specifications

Specifications

1 Kit
Approved for shipment at Room Temperature or on Wet Ice
Blocking Reagent
A kit containing 5 mL each ready-to-use (1X) solutions of reagent A, B, and C in three clear HDPE bottles.
  • Store at 2–8°C.
    • When stored as directed, product can be used for 3 months after receipt.One kit is sufficient to tre
    ReadyProbes™
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    For Research Use Only. Not for use in diagnostic procedures.