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R&D Systems™ Recombinant Human Nectin-4 Protein
Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.
Brand: R&D Systems™ 2659-N4-050
This item is not returnable.
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Description
Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells. When 5 x 104 cells/well are added to recombinant human Nectin-4 coated plates (10 μg/mL with 100 μL/well), approximately >45% will adhere after 1 hour incubation at 37 °C. Optimal dilutions should be determined by each laboratory for each application.Specifications
| NP_112178 | |
| Lyophilized from a 0.2 μm filtered solution in PBS. | |
| M.W. (Observed): 44 kDa, reducing conditions; M.W. (theoretical): 35.6 kDa | |
| 50 μg | |
| Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution. | |
| <0.10 EU / 1 μg of the protein by the LAL method. | |
| Unconjugated | |
| Reconstitute at 200 μg/mL in PBS. | |
| Nectin-4/PVRL4 |
| Bioactivity | |
| 81607 | |
| Nectin-4 | |
| Mouse myeloma cell line, NS0-derived human Nectin-4 protein Gly27-Val351, with a C-terminal 6-His tag | |
| Monomer | |
| NECTIN4 | |
| Recombinant | |
| >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining. |
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