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Ambion™ RiboMinus™ Eukaryote Kit for RNA-Seq

The RiboMinus™ Eukaryote Kit for RNA-Seq provides truly complete transcriptome isolation for next generation sequencing analysis.

Brand:  Ambion™ A1083708

1061.20 EUR valid until 2024-11-30
Use promo code "21651" to get your promotional price.


Product Code. 10063854

  • 1516.00 € / Each

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For Research Use Only. All usage must comply with product instructions.

This item is not returnable. View return policy

Description

Description

The RiboMinus™ Eukaryote Kit for RNA-Seq provides truly complete transcriptome isolation for next generation sequencing analysis by offering a method for selective depletion of up to 99% of the 5S, 5.8S, 18S and 28S ribosomal RNA from total RNA versus poly-A or cap binding methods, which can introduce bias and select for only part of the transcriptome.

The RiboMinus™ Eukaryote Kit for RNA-Seq Offers:
• A simple, yet selective depletion procedure that uses a sequence-specific probe to capture rRNA
• Rapid and efficient removal of rRNA-probe complex using magnetic bead technology

Three Steps to an rRNA-free Preparation Suitable for Transcriptome Analysis
The removal of large ribosomal RNA using the RiboMinus™ kit is a reliable and robust three-step procedure. Hybridize the RNA sample to the RiboMinus™ Probe in a water bath or heat block, then remove probe-rRNA complexes using the RiboMinus™ magnetic beads included in the kit. Concentrate the depleted RNA preparation by ethanol precipitation, or by using the RiboMinus™ Concentration Module (Cat. No. K155005).

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Each kit contains:
• 6 mL RiboMinus™ Magnetic Beads
• 80 μL RiboMinus™ Eukaryote Probe (15 pmol/μL)
• 10 mL Hybridization Buffer
• 12 mL DEPC-treated (RNase-free) water

Store at 4°C.

Total RNA
High-throughput Compatible
8 Preps
1 Each
Wet Ice
Transcriptome RNA
1 μg
Magnetic Bead
Transcriptome RNA
Microarray Analysis, Next-Generation Sequencing, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Sequencing, Northern Blotting, cDNA Library Construction
RiboMinus™
Transcriptome Sequencing
2-10 μg Total RNA
RiboMinus
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For Research Use Only. Not for use in diagnostic procedures.