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Gibco™ RPMI 1640 Medium
RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer.
177.00 €
Specifications
| Content And Storage | Storage conditions: 2-8° C. Protect from light. Shipping conditions: Ambient. Shelf life: 12 months from date of manufacture. |
|---|---|
| Culture Environment | CO2 |
| Cell Type | Mammalian Cells |
| Classification | Animal Origin-free |
| Concentration | 1 X |
Description
RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 Medium modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This RPMI is modified as follows:
With: L-glutamine, Phenol Red
Without: HEPES
The complete formulation is available.
Using RPMI
RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Customers using Gibco™ RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).
Specifications
| Storage conditions: 2-8° C. Protect from light. Shipping conditions: Ambient. Shelf life: 12 months from date of manufacture. |
|
| Mammalian Cells | |
| 1 X | |
| Liquid | |
| Standard Serum Supplementation | |
| Glutamine, Phenol Red, Sodium Bicarbonate | |
| Sustainable packaging | |
| 260 - 310 mOsm/kg | |
| Gibco™ |
| CO2 | |
| Animal Origin-free | |
| Mammalian Cell Culture | |
| RPMI 1640 | |
| Sterile-filtered | |
| No HEPES, No Sodium Pyruvate | |
| cGMP-compliant under the ISO 13485 standard | |
| 7 to 7.4 |
Safety and Handling
missing translation for 'shelfLife' : 12 Months
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.