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Gibco™ RPMI 1640 Medium
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RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer.

177.00 €

Specifications

Content And Storage Storage conditions: 2-8° C. Protect from light.
Shipping conditions: Ambient.
Shelf life: 12 months from date of manufacture.
Culture Environment CO2
Cell Type Mammalian Cells
Classification Animal Origin-free
Concentration 1 X
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Products 1
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
12004997
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Gibco™
21875091
10 x 500 mL
177.00 €
Pack of 10
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Description

Description

RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 Medium modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This RPMI is modified as follows:
With: L-glutamine, Phenol Red
Without: HEPES

The complete formulation is available.

Using RPMI
RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Customers using Gibco™ RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

Specifications

Specifications

Storage conditions: 2-8° C. Protect from light.
Shipping conditions: Ambient.
Shelf life: 12 months from date of manufacture.
Mammalian Cells
1 X
Liquid
Standard Serum Supplementation
Glutamine, Phenol Red, Sodium Bicarbonate
Sustainable packaging
260 - 310 mOsm/kg
Gibco™
CO2
Animal Origin-free
Mammalian Cell Culture
RPMI 1640
Sterile-filtered
No HEPES, No Sodium Pyruvate
cGMP-compliant under the ISO 13485 standard
7 to 7.4
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Safety and Handling

Safety and Handling

missing translation for 'shelfLife' : 12 Months

SDS
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Documents

Product Certifications

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

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