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Thermo Scientific™ Slanetz and Bartley Medium
Detect and enumerate enterococci from water and food samples with Thermo Scientific™ Slanetz and Bartley Medium.
Brand: Thermo Scientific™ PO5018A
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Description
Detect and enumerate enterococci from water and food samples with Thermo Scientific™ Slanetz and Bartley Medium. Slanetz and Bartley1 originally devised this medium to detect and enumerate enterococci by the technique of membrane filtration, but it has also proved useful as a direct plating medium2,3. The medium is very selective for enterococci and, when it is incubated at elevated temperatures (44-45°C), all red or maroon colonies may be accepted as presumptive enterococci4,5.
The Environment Agency ‘Microbiology of Drinking Water 2002’6 recommend the use of Slanetz and Bartley medium for the enumeration of enterococci in water supplies, as does ISO in the standard for water quality7.
The water is filtered through a membrane filter which is then placed on the surface of a well dried plate of the medium. Plates are incubated at 35°C for 4 hours and then at 44-45°C for 44 hours. Membranes are examined, with a hand lens in a good light, and all red or maroon colonies counted as enterococci.
Food samples can be examined for enterococci by the method suggested by the Nordic Committee of Food Analysis3. Samples are homogenizsed and so diluted with physiological saline that only 15 to 150 colonies grow on each Petri dish. Homogenates or dilutions are spread evenly over the agar surface with a glass rod and allowed to soak in. Dishes are inverted and incubated at 35°C for 48 hours, after which typical colonies (pink or dark red, with a narrow whitish border) are counted.
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Specifications
| Beige to beige red, transparent | |
| Slanetz and Bartley Medium | |
| Germany | |
| 17 mL | |
| Flow Wrapped - Single Layer |
| 2° to 12°C, in the dark | |
| 90mm Monoplate | |
| Microbiology Media | |
| 17g ± 5% | |
| 10/Pk. |
For In Vitro Diagnostic Use.