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Molecular Probes™ Streptavidin, Alexa Fluor™ 647 Conjugate
Alexa Fluor™ 647 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor™ dye).
416.00 € - 498.00 €
Specifications
Conjugate | Alexa Fluor 647 |
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Product Line | Alexa Fluor™ |
Product Type | Streptavidin Conjugate (fluorescent) |
Label or Dye | Alexa Fluor |
Product Code | Brand | Form | Quantity | Price | Quantity & Availability | ||||
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Product Code | Brand | Form | Quantity | Price | Quantity & Availability | ||||
10349222
|
Molecular Probes™
S32357 |
Liquid | 0.5 mL |
498.00 €
0.50mL |
In Stock
Log in to see stock. |
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10308062
|
Molecular Probes™
S21374 |
Solid | 1 mg |
416.00 €
1mg |
In Stock
Log in to see stock. |
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Description
Alexa Fluor™ 647 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor™ dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays. Alexa Fluor™ dye streptavidin conjugates are supplied as 1 mg lyophilized product or in 0.5 mL volumes of a 2 mg/mL solution.
Important Features of Alexa Fluor™ 647 Streptavidin Conjugates:
• Alexa Fluor™ 647 streptavidin conjugate has Ex/Em maxima of ∼ (650/668)
• Bright, photostable fluorescence
• High solubility in aqueous solutions
• Available in multiple colors
• Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more
Properties of Alexa Fluor™ Dyes
Alexa Fluor™ dyes are organic fluorescent dyes developed for better performance in imaging and other labeling protocols and exhibit improved photostability and brightness and improved solubility in aqueous solutions. Available in a broad range of colors, these dyes are a good choice for most imaging applications.
Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit (Cat. No. E21390) to minimize this interference.
Consult user Manual for solubility instructions.
Specifications
Alexa Fluor 647 | |
Alexa Fluor™ | |
Streptavidin Conjugate (fluorescent) | |
Alexa Fluor |
For Research Use Only. Not for use in diagnostic procedures.