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Thermo Scientific™ SulfoLink™ Immobilization Kit for Proteins, 2 mL
Covalently immobilize cysteine-peptides or proteins (sulfhydryl-ligands) for affinity purification using iodoacetyl-activated agarose beads and columns.
Brand: Thermo Scientific™ 44995
This item is not returnable.
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Description
Thermo Scientific™ SulfoLink Coupling Resin is iodoacetyl-activated for the covalent immobilization of sulfhydryl-containing peptides or proteins for affinity purification.
SulfoLink Coupling Resin is porous, crosslinked 6% beaded agarose that has been activated with iodoacetyl groups. When incubated with a solution of peptide or protein that contains reduced cysteine residues, the iodoacetyl groups react specifically and efficiently with the exposed sulfhydryls (-SH) to form covalent and irreversible thioether bonds that permanently attach the peptide or protein to the resin. The result is a custom-made affinity resin for purification of antibodies, antigens and other molecules of interest.Complete SulfoLink Immobilization Kits are available for peptides and proteins. Each kit contains all the reagents for preparing the respective peptide or protein sample and five columns containing 2mL of SulfoLink Resin that can be used in either gravity-flow or centrifuge format for efficient coupling reactions and multiple cycles of affinity purification. A single-column Trial Kit for use with either peptides or proteins is also available, or the SulfoLink Coupling Resin can be purchased separately.
Highlights:
Specific conjugation through sulfhydryl (-SH) groups – the iodoacetyl groups react specifically with sulhydryls to form irreversible thioether bonds
Separate kits optimized for peptides or proteins – kits include optimized reagents for preparing peptide or protein samples for efficient immobilization
Fast – spin columns increase protocol speed; prepare and couple samples in 2 hours (peptides) to 3.5 hours (proteins)
Flexible coupling conditions – use pH 7.5 to 9.0 aqueous buffers, organic solvent (e.g., 20% DMSO) or denaturant (guanidine·HCl), as needed for protein or peptide solubility during coupling reaction
Easy-to-follow instructions – streamlined protocols for sample preparation, immobilization and affinity purification
High capacity – immobilize 1 to 2mg peptide or 2 to 20mg protein per 2mL column of SulfoLink Coupling Resin
Includes:
Kits contain SulfoLink Resin Columns (2mL), protein or peptide preparation reagents (e.g., reducing agent), coupling buffer, wash solution, L-cysteine solution (for quenching) and desalting columns (protein kit only)
Recommended for:
Immobilize peptides having terminal cysteine residues to purify antibodies that were generated against peptide immunogens prepared by maleimide conjugation; Immobilize antibodies in oriented manner through hinge-region sulfhydryls to ensure that antigen binding sites are not sterically hindered for antigen affinity purification
Specifications
| 2 mL | |
| SulfoLink™ | |
| Immobilization Kit | |
| 2 to 20 mg of Antibody per Column | |
| Affinity, Agarose Resin |
| Sufficient For: Immobilizing 1 to 10 mg of antibody per column • SulfoLink Columns, 2 mL, 5 columns • SulfoLink Preparation Buffer, 7.5 mL • SulfoLink Coupling Buffer, 500 mL • Wash Solution, 120 mL • PBS Pack (makes 500 mL), 1 pack • 2-Mercaptoethylamine·HCl, 5 x 6 mg • L-Cysteine·HCl, 100 mg • Zeba Spin Desalting Columns, 5 mL, 5 columns • Column Accessories Store at 4°C protected from light. |
|
| 5 Column Kit | |
| Haloacetyl | |
| Spin Column |
For Research Use Only. Not for use in diagnostic procedures.