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Invitrogen™ SuperScript™ IV One-Step RT-PCR System
The Invitrogen SuperScript One-Step RT-PCR System, available with or without ezDNase, combines the high processivity of SuperScript IV Reverse Transcriptase (RT) with the high-fidelity of Platinum SuperFi DNA Polymerase to provide unmatched one-step RT-PCR performance.
385.00 € - 1216.00 €
Specifications
Detection Method | Gel Electrophoresis |
---|---|
Format | Kit |
GC-Rich PCR Performance | High |
PCR Method | 1-step RT-PCR |
Polymerase | Platinum SuperFi |
Includes:
- SuperScript IV RT Mix, 12.5µL
- 2X Platinum SuperFi RT-PCR Master Mix, 625µL
- Nuclease-free water, 1.25mL
Product Code | Brand | Includes | No. of Reactions | Price | Quantity & Availability | ||||
---|---|---|---|---|---|---|---|---|---|
Product Code | Brand | Includes | No. of Reactions | Price | Quantity & Availability | ||||
15814182
|
Invitrogen™
12594025 |
Kit only | 25 Reactions |
385.00 €
25 reactions |
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15781999
|
Invitrogen™
12594100 |
Kit only | 100 Reactions |
1094.00 €
Each |
Please sign in to purchase this item. Need a web account? Register with us today! | ||||
15895891
|
Invitrogen™
12595025 |
Kit with ezDNase | 25 Reactions |
414.00 €
25 reactions |
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15850233
|
Invitrogen™
12595100 |
Kit with ezDNase | 100 Reactions |
1216.00 €
100 reactions |
Please sign in to purchase this item. Need a web account? Register with us today! | ||||
Description
The Invitrogen SuperScript One-Step RT-PCR System, available with or without ezDNase, combines the high processivity of SuperScript IV Reverse Transcriptase (RT) with the high-fidelity of Platinum SuperFi DNA Polymerase to provide unmatched one-step RT-PCR performance. With this convenient one-step formulation plus gene-specific primers, you can perform both cDNA synthesis from total RNA or mRNA and PCR amplification in a single tube.
Features of the SuperScript IV One-Step RT-PCR System include:
• Superior performance with sensitivity down to 0.01 pg RNA, target length up to 13.8 kb, and the fastest one-step RT-PCR protocol
• Reliable target detection even with suboptimally pure RNA samples
• Two-phase hot-start activation mechanism for high sensitivity, specificity, and room temperature setup
• Fast and easy gDNA removal for superior accuracy and confidence in results (SuperScript IV One-Step RT-PCR System with ezDNase only)
SuperScript IV RT is a proprietary MMLV RT mutant with increased processivity, thermostability, and inhibitor resistance, as well as reduced RNase H activity, for sensitive and efficient full-length cDNA synthesis. Platinum SuperFi DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum hot-start technology for the highest success in PCR. The combination of these two engineered enzymes produces enhanced RT-PCR yields and fidelity, as well as amplification of longer templates compared to other one-step RT-PCR kits.
The inclusion of ezDNase (SuperScript IV One-Step RT-PCR System with ezDNase only) for use upstream of the RT step further enhances the one-step RT-PCR workflow through an extremely simplified genomic DNA removal step that ensures the highest accuracy and confidence in one-step RT-PCR results.
Specifications
Gel Electrophoresis | |
High | |
Platinum SuperFi | |
1-step RT-PCR | |
SuperScript IV | |
Dry Ice | |
Built-In Hot Start | |
Reverse Transcription | |
RNA |
Kit | |
1-step RT-PCR | |
10 min. | |
45°C to 60°C | |
Reduced | |
PCR Amplified cDNA | |
Master Mix | |
Up to 14 kb |
For Research Use Only. Not for use in diagnostic procedures.
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