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Invitrogen™ SuperScript™ VILO™ Master Mix

SuperScript VILO Master Mix is a first-strand cDNA synthesis master mix for two-step RT-qPCR applications.

Brand: Invitrogen™ 11755250

2481.50 EUR valid until 2024-12-31
Use promo code "21651" to get your promotional price.

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Product Code. 12023679

3545.00 € / 250 reactions

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For Research Use Only. All usage must comply with product instructions.

This item is not returnable. View return policy

Description

SuperScript VILO Master Mix is a first-strand cDNA synthesis master mix for two-step RT-qPCR applications. The master mix combines SuperScript III Reverse Transcriptase (RT) and other RT reaction components in a single-tube format to enable reliable first-strand synthesis and high cDNA yields from a wide range of input RNA concentrations. The high RT efficiency of SuperScript III RT and the optimized formulation allow the master mix to deliver high linearity in cDNA synthesis across a broad range of input RNA template for two-step quantitative RT-PCR assays.

Features of SuperScript VILO Master Mix include:
• Starting material can range up to 2.5 μg of total RNA in a 20 μL cDNA synthesis reaction
• Recombinant ribonuclease inhibitor safeguards against the degradation of target RNA due to ribonuclease contamination
• RT is engineered with reduced RNase H activity and increased thermal stability

For superior cDNA synthesis performance in RT-qPCR applications
SuperScript IV VILO Master Mix (Cat. No. 11756050) elevates the trusted VILO technology to the next level with the highly processive and thermostable SuperScript IV RT and further optimized buffer. The master mix enables efficient cDNA synthesis at higher temperatures in less time and provides superior cDNA yield and sensitivity even with suboptimal purity or scarce templates.

Specifications

Content And Storage	• SuperScript VILO Master Mix (1 mL) Store at –5 to –30°C.
Format	Master Mix
Reaction Speed	60 min.
Optimal Reaction Temperature	42°C
Reverse Transcriptase	SuperScript III
Shipping Condition	Dry Ice
Concentration	5X
No. of Reactions	250 Reactions
Reagent Type	Reverse Transcription
Starting Material	RNA

Detection Method	qPCR
GC-Rich PCR Performance	High
Technique	Reverse Transcription
Quantity	250 reactions
Ribonuclease H Activity	Reduced
For Use With (Application)	Real Time PCR (qPCR)
Final Product Type	First-Strand cDNA
Reaction Format	Master Mix
Size (Final Product)	Up to 10 kb

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Invitrogen™ SuperScript™ VILO™ Master Mix

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