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Thermo Scientific™ T4 DNA Ligase (5 U/μL)
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.
Brand: Thermo Scientific™ EL0012
227.03 EUR valid until 2024-12-31
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This item is not returnable.
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Description
Catalyze the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA with the Thermo Scientific™ T4 DNA Ligase. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids. It also joins DNA fragments with either cohesive or blunt termini, but has no activity on single-stranded nucleic acids.
The T4 DNA Ligase requires ATP as a cofactor.
Highlights
- Active in Themo Scientific restriction enzyme, PCR, and RT buffers (when supplemented with ATP)
- Fast – sticky-end ligation is completed in 10 minutes at room temperature
- Supplied with PEG solution for efficient blunt-end ligation
Applications
- Cloning of restriction enzyme generated DNA fragments
- Cloning of PCR products
- Joining of double-stranded oligonucleotide linkers or adaptors to DNA
- Site-directed mutagenesis
- Amplified fragment length polymorphism (AFLP)
- Ligase-mediated RNA detection (see Reference 3)
- Nick repair in duplex DNA, RNA or DNA/RNA hybrids
- Self-circularization of linear DNA.
Notes
- Binding of T4 DNA Ligase to DNA may result in a band shift in agarose gels. To avoid this, incubate samples with 6X DNA Loading Dye & SDS Solution at 70°C for 5 min or 65°C for 10 minutes and chill on ice prior to electrophoresis.
- The volume of the ligation reaction mixture should not exceed 10% of the competent cell volume in the transformation process.
- Prior to electro-transformation, remove T4 DNA Ligase from the ligation mixture by spin column or chloroform extraction. The extracted DNA can be further precipitated with ethanol.
Specifications
| 5 U/μL | |
| 10X T4 DNA Ligase Buffer | |
| T4 DNA Ligase |
| DNA Ligase | |
| 5 x 1,000 units |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- T4 DNA Ligase (5 U/µL)
Signal Word
- Warning
Hazard Category
- Serious eye damage/eye irritation Category 2
Hazard Statement
- H319-Causes serious eye irritation.
Precautionary Statement
- P264-Wash hands thoroughly after handling.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P337+P313-If eye irritation persists: Get medical advice/attention.
Supplemental information
- MIXTURE LIST-Contains : (R*,R*)-1,4-dimercapto-2,3-butanediol
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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