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TaqMan™ TaqMan™ Genotyping Master Mix

Optimized for end-point fluorescence detection in SNP genotyping applications.

Brand: TaqMan™ 4371353

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Product Code. 10390065

142.00 € / Each

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For Research Use Only. All usage must comply with product instructions.

This item is not returnable. View return policy

Description

Distinct clusters and high call rates for unambiguous allelic discrimination
Validated with TaqMan™ SNP Genotyping Assays
Excellent pre- and post-PCR stability for high throughput setup and analysis
Reaction size is based on a 50μL reaction volume

May be used for a variety of genotyping applications, including:

Candidate gene studies
Drug-target validation
Disease association studies
Population genetics
Linkage mapping
Agricultural applications

Exceptional Performance from an Optimized Mix (2X)
Accurate genotype assignments result from preferential binding of the allele-specific probe to the matching target. TaqMan™ Genotyping Master Mix enables specific binding of the probe to achieve exceptional cluster resolution. Key features include:

AmpliTaq Gold™ DNA Polymerase, UP (Ultra Pure) - Automatic hot start enzyme designed to be active during thermal cycling and inactive at room temperature for easy reaction setup
Optimized mix components provide excellent specificity for discrimination between alleles
Passive internal reference based on proprietary ROX™ dye for increased precision on Applied Biosystems™ real-time PCR instruments
Single thermal cycling condition for consistent results with TaqMan™ Assays

Validated with TaqMan™ Genotyping Assays and Instruments:

TaqMan™ Genotyping Master Mix has been tested across all types of TaqMan™ SNP Genotyping Assays: TaqMan™ Drug Metabolism Genotyping Assays, TaqMan™ SNP Genotyping Assays, and Custom TaqMan™ SNP Genotyping Assays
In addition, the master mix is validated with the Applied Biosystems™ thermal cyclers and real-time PCR systems

High-Throughput Setup and Analysis:

The combination of several low-cost thermal cyclers for PCR and a single real-time PCR instrument for allelic discrimination make high throughput SNP genotyping manageable
TaqMan™ Genotyping Master Mix further improves high-throughput setup and analysis with excellent room temperature stability both before and after PCR
This provides the flexibility required to run long experiments, unattended overnight or over a weekend

Reliable Discrimination with Challenging Targets

Amplicon design constraints make SNP detection challenging, particularly when targets contain an abundance of GC- or AT-rich regions
The optimized components of TaqMan™ Genotyping Master Mix result in tight, well-separated clusters and more accurate allele-calls compared to other mixes, even for challenging targets

Setting the Industry Standard for Quality:

Extensive analytical and functional tests are performed for each manufactured lot of TaqMan™ Genotyping Master Mix, including one TaqMan™ SNP Genotyping Assay and one TaqMan™ Drug Metabolism Genotyping Assay
Results are compiled in an informative Certificate of Analysis, which is accessible through the Applied Biosystems™ Web site

Order Info

Guaranteed minimum shelf life is 60 days. Shipping Conditions: Wet Ice

Specifications

Content And Storage	Supplied at 2X concentration. The mix is optimized for SNP genotyping using TaqMan™ probes and contains AmpliTaq Gold™ DNA Polymerase UP (Ultra Pure), dNTPs without dUTP, Passive Reference 1 and optimized mix components. This pack contains one 1 mL tube, sufficient for 40 reactions at the 50 μL total volume. Storage: 2-8°C Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
GC-Rich PCR Performance	High
Polymerase	AmpliTaq Gold DNA Polymerase
Technique	SNP Genotyping (Real-Time PCR-Based), CNV (Copy Number Variation)
Genotyping Target	Copy Number Variants, SNPs (Unknown or Numerous), Drug Metabolizing Enzymes, SNPs (Known)
Product Line	TaqMan™
Purity or Quality Grade	UP (Ultra Pure)
For Use With (Application)	Genotyping
Fidelity (vs. Taq)	2 X
Volume	1 mL

Detection Method	Primer-probe
PCR Method	qPCR
Reaction Speed	Standard
For Use With (Equipment)	Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7
Passive Reference Dye	ROX (Pre-mixed)
Product Type	Genotyping Master Mix
Quantity	1 x 1 mL
Concentration	2X
Sample Type	DNA (Genomic)

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Documents

Scientific Resources

Brochures

Essentials of Real Time PCR File size: 153.6 kB

Quick Reference Card: TaqMan® Genotyping Master Mix File size: 53.2 kB

Product Information

Manuals

Product Sheet: TaqMan Genotyping Master Mix Version: D File size: 126.3 kB

Protocol: TaqMan® Genotyping Master Mix: Protocol (English ) Version: 19 Aug 2010 File size: 1 MB

Warranties

Product Bulletin: TaqMan® SNP Genotyping Assays File size: 642.6 kB

Product Bulletin: TaqMan Genotyping Master Mix File size: 1 MB

Certificates

A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.

Lot Number

5 results found

Lot Number	Certificate Type	Date	Catalog Number
Lot Number2800847	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog Number4371353
Lot Number2773396	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog Number4371353
Lot Number2831546	Certificate TypeCertificate of Analysis	Date28/01/2025	Catalog Number4371353
Lot Number2870979	Certificate TypeCertificate of Analysis	Date28/01/2025	Catalog Number4371353
Lot Number2874225	Certificate TypeCertificate of Analysis	Date28/01/2025	Catalog Number4371353

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