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Thermo Scientific™ Luminaris HiGreen qPCR Master Mix, high ROX

Product Code. p-4861498 Shop All Thermo Scientific Products
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No. of Reactions:
1250 Reactions
250 Reactions
500 Reactions
5000 Reactions
Unit Size:
1250 reactions
250 reactions
500 reactions
5000 reactions
This item is not returnable. View return policy

Product Code. 13575250

Brand: Thermo Scientific™ K0963

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Includes

Luminaris HiGreen High ROX qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG, dNTPs (with dUTP) and SYBR Green I in an optimized PCR buffer. The master mix contains high concentration of ROX passive reference dye for specific real-

Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR.

  • UDG in the master mix to prevent carry-over contamination
  • Equal performance of blue and colorless master mixes
  • Versions with premixed ROX or fluorescein for different real-time platforms
  • Standard cycling protocols
  • Excellent qPCR performance
  • Specificity: Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
  • Sensitivity: ingle copy detection
  • Wide linear range: accurate quantification across 9 orders of magnitude
  • Reproducibility and convenience: ready-to-use 2X master mix
TRUSTED_SUSTAINABILITY

Specifications

Concentration 2X
Content And Storage Contains:
• 10 x 1.25 mL Luminaris HiGreen High ROX qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG, dNTPs (with dUTP) and SYBR Green I in an optimized PCR buffer; sufficient for 1250 x 20 μL reactions. The master mix contains high concentration of ROX passive reference dye for specific real-time platforms.
• 10 x 1.25 mL Nuclease-Free Water

Store at -20°C.
Detection Method SYBR
GC-Rich PCR Performance High
PCR Method qPCR
Polymerase Taq DNA Polymerase
Reaction Speed Standard
For Use With (Equipment) 7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
Product Line Luminaris
Product Type qPCR Master Mix
Quantity 1250 Reactions
Sufficient For 250 Reactions
For Use With (Application) Gene Expression
Fidelity (vs. Taq) 2 X
No. of Reactions 1250 Reactions
Sample Type DNA, dsDNA
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For Research Use Only. Not for use in diagnostic procedures.

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