Thermo Scientific™ 96-Well Plates for Pierce™ Quantitative Fluorometric Peptide Assay

Description

The Pierce Quantitative Fluorescent Peptide Assay reagents include peptide assay buffer, fluorescent peptide labeling reagent, and a peptide digest assay standard for the quantitative measurement of peptide concentrations. In this assay, peptides are specifically labeled at the amino-terminus using an amine-reactive fluorescent reagent, and the fluorescently labeled peptides are detected using 390 nm (excitation) and 475 nm (emission).

The Thermo Scientific Pierce Quantitative Fluorescent Peptide Assay is a sensitive, mix-and-read fluorescent microplate assay for the quantitative measurement of peptides and peptide mixtures.

• Sensitive - accurately detect as little as 5.0μg/mL of single peptides or peptide mixtures
• Robust - assay performance rigorously tested using both peptides and peptide digest mixtures
• Robust peptide digest standard - kit includes a validated peptide digest standard for improved reproducibility of quantitation
• Compatible - works with many reagents, including those used in mass spectrometry sample preparation
• Convenient - easy mix-and-read format and stable fluorescent signal that may be read in as little as 5 minutes up to several hours

Because of the labeling mechanism of the fluorescent assay reagent, this assay is suitable for the quantitative measurement of synthetic peptides as well as peptide digest mixtures. A high quality peptide digest reference standard is included for use in generating linear standard curves and as a calibration control for improved accuracy and reproducibility. This sensitive assay requires only 10 μL of sample, produces a linear response with increasing peptide concentrations (5 to 1000 μg/mL), and results in a stable final fluorescence that can be detected in as little as five minutes.

Applications:

• Recommended for use with peptide mixtures and synthetic peptides with an unblocked amino-terminus (amino-terminus-labeled peptides, such as TMT-labeled peptides, are not compatible with this assay)
• Normalizing sample concentrations for quantitative downstream applications
• Normalizing sample loading volumes for LC, MS, and LC/MS applications
• Measuring recovery after peptide clean-up procedures