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Thermo Scientific™ RevertAid RT Reverse Transcription Kit

The Thermo Scientific RevertAid RT Kit is a complete system for efficient synthesis of first strand cDNA from mRNA or total RNA templates.

Brand: Thermo Scientific™ K1691

667.80 EUR valid until 2024-12-31
Use promo code "21651" to get your promotional price.

Product Code. 15255146

954.00 € / 500 reactions

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This item is not returnable. View return policy

Description

The Thermo Scientific RevertAid RT Kit is a complete system for efficient synthesis of first strand cDNA from mRNA or total RNA templates. The kit uses RevertAid Reverse Transcriptase (RT), which has lower RNase H activity compared to AMV reverse transcriptase. The enzyme maintains activity at 42–50°C and is suitable for synthesis of cDNA up to 13 kb. The recombinant RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA from degradation at temperatures up to 55°C.

First strand cDNA synthesized with this system can be directly used as a template in PCR or real-time PCR. It is also ideal as a first step for second strand cDNA synthesis or linear RNA amplification. Radioactively and non-radioactively labeled nucleotides can be incorporated into first strand cDNA for use as a probe in hybridization experiments, including microarrays.

Features of the RevertAid RT Kit include:
• Full-length first strand cDNA up to 13 kb
• Optimum reaction temperature 42°C
• Complete kit includes all the components for RT reactions

Applications
• First strand cDNA synthesis for RT-PCR and RT-qPCR
• Construction of full length cDNA libraries
• Antisense RNA synthesis

Order Info

Orders placed Monday through Wednesday ship next day; Thursday and Friday orders ship the following Monday.

Specifications

Content And Storage	• RevertAid Reverse Transcriptase • Ribolock RNase Inhibitor • 5X Reaction Buffer • dNTP Mix • Random Hexamer Primer • Nuclease-free water Store at –20°C.
Reaction Speed	60 min.
Optimal Reaction Temperature	42°C
Reverse Transcriptase	RevertAid
Shipping Condition	Dry Ice
Final Product Type	First-Strand cDNA
Reaction Format	Separate components
Size (Final Product)	Up to 13 kb

Format	Kit
Technique	Reverse Transcription
Quantity	500 reactions
Ribonuclease H Activity	Yes
For Use With (Application)	Real Time PCR (qPCR)
No. of Reactions	500 Reactions
Reagent Type	Reverse Transcription
Starting Material	RNA

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Thermo Scientific™ RevertAid RT Reverse Transcription Kit

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