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Invitrogen™ TOP10 Electrocomp™ Kit

TOP10 Electrocomp E. coli cells are provided at a transformation efficiency of 1 x 1010 cfu/μg supercoiled DNA and are ideal for high-efficiency cloning and plasmid propagation.

Brand:  Invitrogen™ C66455

241.68 EUR valid until 2024-12-31
Use promo code "21651" to get your promotional price.

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Product Code. 10398162

  • 318.00 € / Each

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For Research Use Only. All usage must comply with product instructions.

This item is not returnable. View return policy

Description

Description

TOP10 Electrocomp E. coli cells are provided at a transformation efficiency of 1 x 1010 cfu/μg supercoiled DNA and are ideal for high-efficiency cloning and plasmid propagation. This strain is used in wide range of applications and allows stable replication of high-copy number plasmids and maintenance of large plasmids. 

The TOP10 genotype is similar to the DH10B strain and features:
• hsdR for efficient transformation of unmethylated DNA from PCR amplifications
• mcrA for efficient transformation of methylated DNA from genomic preparations
• lacZΔM15 for blue/white color screening of recombinant clones
• endA1 for cleaner preparations of DNA and better results in downstream applications due to the elimination of non-specific digestion by Endonuclease I
• recA1 for reduced occurrence of non-specific recombination in cloned DNA

Notes
Please see the product manual for reaction scale recommendations. A high-voltage electroporation apparatus is required.

Genotype
F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL endA1 nupG

Find the strain and format that fits your needs
• TOP10 is available in chemically competent and electrocompetent cell formats.
• Choose MultiShot competent cells for high-throughput cloning applications.

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Electrocompetent Cells
Yes (endA1)
High Efficiency (>1 x 1010 cfu/μg)
Yes (Streptomycin)
Yes (lacΔM15)
May be used for plasmids >20 kb
Yes (recA1)
No
Tube
5 x 100 μL
No
Yes (mcrA)
• 5 x 100μL TOP10 Electrocomp E. coli
• 50 μL pUC19 vector (10 pg/μL)
Store at –80°C.

• S.O.C. Medium (15 mL)
Store at room temperature.
Not suitable for cloning unstable DNA
Low
No
Dry Ice
E. coli (K12)
Electrocomp™
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For Research Use Only. Not for use in diagnostic procedures.